ArticlePloS one2013
Enhanced transgene expression in sugarcane by co-expression of virus-encoded RNA silencing suppressors.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Tomato Bushy Stunt Virus (TBSV): From a Plant Pathogen to a Multifunctional Biotechnology Platform.Viruses · 2025Review
- RNAi and genome editing of sugarcane: Progress and prospects.The Plant journal : for cell and molecular biology · 2025Review
- Identification of a sugarcane bacilliform virus promoter that is activated by drought stress in plants.Communications biology · 2024Article
- Plant-based expression platforms to produce high-value metabolites and proteins.Frontiers in plant science · 2022Review
- Functional Characterization of Genes Coding for NovelFrontiers in plant science · 2022Article
- Genetic transformation technologies for the common dandelion, Taraxacum officinale.Plant methods · 2021Article
- RNA silencing suppressor-influenced performance of a virus vector delivering both guide RNA and Cas9 for CRISPR gene editing.Scientific reports · 2021Article
- Genetic Transformation of Sugarcane, Current Status and Future Prospects.Frontiers in plant science · 2021Review
- A Sugarcane G-Protein-Coupled Receptor,Frontiers in plant science · 2021Article
- High-Level Production of Recombinant Snowdrop Lectin in Sugarcane and Energy Cane.Frontiers in bioengineering and biotechnology · 2020Article
- Transient plant production ofBiotechnology reports (Amsterdam, Netherlands) · 2019Article
- Manipulating plant RNA-silencing pathways to improve the gene editing efficiency of CRISPR/Cas9 systems.Genome biology · 2018Article
- A biolistic-based genetic transformation system applicable to a broad-range of sugarcane and energycane varieties.GM crops & food · 2018Article
- Multiplexed Gene Editing and Protein Overexpression Using aPlant physiology · 2017Article
- Cytosolic delivery of siRNA by ultra-high affinity dsRNA binding proteins.Nucleic acids research · 2017Article
- Reproducible genomic DNA preparation from diverse crop species for molecular genetic applications.Plant methods · 2017Article
- A novelBiotechnology for biofuels · 2017Article
- Article
- Physiological and transcriptional analyses of developmental stages along sugarcane leaf.BMC plant biology · 2015Article
- Epitope-tagged protein-based artificial miRNA screens for optimized gene silencing in plants.Nature protocols · 2014Article
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Authors and funding
13 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Post-transcriptional gene silencing is commonly observed in polyploid species and often poses a major limitation to plant improvement via biotechnology. Five plant viral suppressors of RNA silencing were evaluated for their ability to counteract gene silencing and enhance the expression of the Enhanced Yellow Fluorescent Protein (EYFP) or the β-glucuronidase (GUS) reporter gene in sugarcane, a major sugar and biomass producing polyploid. Functionality of these suppressors was first verified in Nicotiana benthamiana and onion epidermal cells, and later tested by transient expression in sugarcane young leaf segments and protoplasts. In young leaf segments co-expressing a suppressor, EYFP reached its maximum expression at 48-96 h post-DNA introduction and maintained its peak expression for a longer time compared with that in the absence of a suppressor. Among the five suppressors, Tomato bushy stunt virus-encoded P19 and Barley stripe mosaic virus-encoded γb were the most efficient. Co-expression with P19 and γb enhanced EYFP expression 4.6-fold and 3.6-fold in young leaf segments, and GUS activity 2.3-fold and 2.4-fold in protoplasts compared with those in the absence of a suppressor, respectively. In transgenic sugarcane, co-expression of GUS and P19 suppressor showed the highest accumulation of GUS levels with an average of 2.7-fold more than when GUS was expressed alone, with no detrimental phenotypic effects. The two established transient expression assays, based on young leaf segments and protoplasts, and confirmed by stable transgene expression, offer a rapid versatile system to verify the efficiency of RNA silencing suppressors that proved to be valuable in enhancing and stabilizing transgene expression in sugarcane.
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