ArticlePloS one2013
Gene expression profiling specifies chemokine, mitochondrial and lipid metabolism signatures in leprosy.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it, 58 citations in OpenAlex.
- An update on leprosy immunopathogenesis: systematic review.Frontiers in immunology · 2024Pooled it
- Functional single nucleotide polymorphisms in CACNA2D3 and other autophagy-related genes are associated with leprosy among Brazilians.PLoS neglected tropical diseases · 2026Article
- The Sm29 antigen differentially shapes transcriptomic and regulatory landscapes across reactional forms of leprosy.Frontiers in immunology · 2026Article
- Article
- Decidual production of interferon lambda in response to ZIKV persistence: Clinical evidence andHeliyon · 2024Article
- High-Resolution Plasma Metabolomics Identifies Alterations in Fatty Acid, Energy, and Micronutrient Metabolism in Adults Across the Leprosy Spectrum.The Journal of infectious diseases · 2024Article
- Immunomodulatory effect of bovine lactoferrin during SARS-CoV-2 infection.Frontiers in immunology · 2024Article
- Article
- Whole mitogenome sequencing uncovers a relation between mitochondrial heteroplasmy and leprosy severity.Human genomics · 2023Article
- What happens when Schwann cells are exposed toIBRO neuroscience reports · 2023Review
- Reanalysis and validation of the transcriptional pleural fluid signature in pleural tuberculosis.Frontiers in immunology · 2023Article
- Cutting edge medical research.Medical journal, Armed Forces India · 2022Article
- Mitochondria inInternational journal of molecular sciences · 2022Review
- Gene expression patterns associated with multidrug therapy in multibacillary leprosy.Frontiers in cellular and infection microbiology · 2022Article
- Article
- Putative pathogen-selected polymorphisms in the PKLR gene are associated with mycobacterial susceptibility in Brazilian and African populations.PLoS neglected tropical diseases · 2021Article
- Blood RNA signature RISK4LEP predicts leprosy years before clinical onset.EBioMedicine · 2021Article
- In search of biomarkers for leprosy by unraveling the host immune response to Mycobacterium leprae.Immunological reviews · 2021Review
- Reduction of host cell mitochondrial activity as Mycobacterium leprae's strategy to evade host innate immunity.Immunological reviews · 2021Review
- Mitochondria: Powering the Innate Immune Response to Mycobacterium tuberculosis Infection.Infection and immunity · 2021Review
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Herein, we performed microarray experiments in Schwann cells infected with live M. leprae and identified novel differentially expressed genes (DEG) in M. leprae infected cells. Also, we selected candidate genes associated or implicated with leprosy in genetic studies and biological experiments. Forty-seven genes were selected for validation in two independent types of samples by multiplex qPCR. First, an in vitro model using THP-1 cells was infected with live Mycobacterium leprae and M. bovis bacillus Calmette-Guérin (BCG). In a second situation, mRNA obtained from nerve biopsies from patients with leprosy or other peripheral neuropathies was tested. We detected DEGs that discriminate M. bovis BCG from M. leprae infection. Specific signatures of susceptible responses after M. leprae infection when compared to BCG lead to repression of genes, including CCL2, CCL3, IL8 and SOD2. The same 47-gene set was screened in nerve biopsies, which corroborated the down-regulation of CCL2 and CCL3 in leprosy, but also evidenced the down-regulation of genes involved in mitochondrial metabolism, and the up-regulation of genes involved in lipid metabolism and ubiquitination. Finally, a gene expression signature from DEG was identified in patients confirmed of having leprosy. A classification tree was able to ascertain 80% of the cases as leprosy or non-leprous peripheral neuropathy based on the expression of only LDLR and CCL4. A general immune and mitochondrial hypo-responsive state occurs in response to M. leprae infection. Also, the most important genes and pathways have been highlighted providing new tools for early diagnosis and treatment of leprosy.
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Registered trials
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