ArticleJournal of research in medical sciences : the official journal of Isfahan University of Medical Sciences2012
Development of PCR-based method for detection of Enterobacteriaceae in septicemia.
Article in Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 18 citations in OpenAlex.
- Levels of Different Microbial Groups on Inert Surfaces of Poultry Slaughterhouses: Identification Using Matrix-Assisted Laser Desorption Ionization Time-of-Flight and Detection of Extended-Spectrum Beta-Lactamase- and Carbapenemase-Producing Enterobacteria.Antibiotics (Basel, Switzerland) · 2024Article
- Comparison between Polymerase Chain Reaction and Blood Culture for Diagnosis of Neonatal Sepsis.Archives of Razi Institute · 2023Article
- Cross-Over Pathogenic Bacteria Detected in Infected Tomatoes (Pathogens (Basel, Switzerland) · 2022Article
- Prevalence of Enterobacteriaceae spp. and its multidrug-resistant rates in clinical isolates: A two-center cross-sectional study.Molecular biology reports · 2021Article
- Bogotá River anthropogenic contamination alters microbial communities and promotes spread of antibiotic resistance genes.Scientific reports · 2019Article
- Determination of virulence determinants ofGastroenterology and hepatology from bed to bench · 2019Article
- Detection of plasmid-mediated quinolone resistance in clinical isolates ofSaudi journal of biological sciences · 2018Article
- Biohydrogen production from sugarcane bagasse hydrolysate: effects of pH, S/X, FeEnvironmental science and pollution research international · 2017Article
- Conventional, molecular methods and biomarkers molecules in detection of septicemia.Advanced biomedical research · 2015Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveSepsis is a systemic inflammatory response associated with high mortality rates in the clinical setting. A multiplex endpoint polymerase chain reaction (PCR) based assay for rapid detection of enterobacteriaceae involved in septicemia, which included Internal Control (IC) and 16S rDNA, is presented here. To develop a panel of primers for DNA fragments of 16S rDNA, enterobacteriaceae, IC, and evaluate analytical sensitivity and specificity of the test. MATERIALS AND
methodsPrimers for amplification of enterobacteriaceae, IC, and16S rDNA were designed, and then PCR was performed. Minimal analytical sensitivity was determined by cloning and colony PCR, and specificity was tested on the basis of their respective standard strains. This study is a cross-sectional Model.
resultsOur results showed the rpoB gene as the most promising target for detection of enterobacteriaceae by PCR amplification. Specificity and sensitivity of endpoint PCR were 100%, 100%, and 100%, and 10, 1, and 100 copies/reaction for enterobacteriaceae, IC, and 16S rDNA, respectively.
conclusionThe molecular panel presented offers the advantage of an easy, reliable, and cost-effective system when compared to other molecular detection methods. However, further evaluation is needed. Our assay holds promising for more rapid pathogens related in clinical sepsis.
Indexed as
Identifiers
23798929PMC3685785W1835736326What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.