ArticleProceedings of the National Academy of Sciences of the United States of America2013
Vibrational imaging of newly synthesized proteins in live cells by stimulated Raman scattering microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers, 1 of them a synthesis that pooled it.
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Who cites it
106 citing papers in PubMed, 1 synthesis or guideline pooled it, 239 citations in OpenAlex.
- Discussion on the comparison of Raman spectroscopy and cardiovascular disease-related imaging techniques and the future applications of Raman technology: a systematic review.Lasers in medical science · 2025Pooled it
- Longitudinal live-cell chemical imaging using coherent Raman scattering microscopy.The Analyst · 2026Review
- Biomolecular condensates can function as inherent catalysts.Molecular cell · 2026Article
- Nascent extracellular matrix: the missing piece in hydrogel design.Cell biomaterials · 2026Article
- Raman Spectroscopy in Cancer Diagnostics and Surgery: 25 Years of Progress from Surface-Enhanced Raman Spectroscopy to Artificial Intelligence─A Bibliometric and Visualized Study.Analytical chemistry · 2026Review
- The Next Frontier in Vibrational Imaging: Chemically Designed Raman Probes Pass the Spectral Barrier.JACS Au · 2026Review
- Visualization of a Bruton's Tyrosine Kinase Inhibitor Using Fluorescence and Raman Microscopy.Analytical chemistry · 2026Article
- Surface-enhanced stimulated Raman scattering and fluorescence probing of plasmonic nanoparticles in cellular environments: insights into their spatial distribution and aggregation.Nanoscale advances · 2026Article
- Far-field single-molecule vibrational spectroscopy and imaging.Chemical science · 2026Review
- Raman imaging of molecular groups in the wavenumber silent region.Analytical and bioanalytical chemistry · 2026Review
- Stimulated Raman Scattering Imaging Enabled Biomarker Discovery for Precision Medicine.Chemical & biomedical imaging · 2025Review
- Recent advances in Raman probes for multiplexed bioimaging.Inflammation and regeneration · 2025Review
- Engineering Nanomaterials for Raman Bioimaging and Theranostics: Advances, Challenges, and Future Directions.ACS omega · 2025Review
- Imaging Cellular Metabolic Rewiring with SuMMIT-SRS.bioRxiv : the preprint server for biology · 2025Article
- Deuterium-oxide-assisted stimulated Raman scattering microscopy: metabolic imaging for precision medicine.Biophotonics discovery · 2025Review
- Unmixing Hyperspectral SRS Images in the Cell-Silent Region of the Raman Spectrum Using Phasor Analysis.Chemical & biomedical imaging · 2025Article
- Self-Supervised Elimination of Non-Independent Noise in Hyperspectral Imaging.Newton ((New York, N.Y.) · 2025Article
- Condenzymes: Biomolecular condensates with inherent catalytic activities.bioRxiv : the preprint server for biology · 2025Article
- Illuminating life processes by vibrational probes.Nature methods · 2025Review
- Enzyme-Instructed Self-Assembly Reprograms Fatty Acid Metabolism for Cancer Therapeutics.Advanced healthcare materials · 2025Article
46 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
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Abstract
Synthesis of new proteins, a key step in the central dogma of molecular biology, has been a major biological process by which cells respond rapidly to environmental cues in both physiological and pathological conditions. However, the selective visualization of a newly synthesized proteome in living systems with subcellular resolution has proven to be rather challenging, despite the extensive efforts along the lines of fluorescence staining, autoradiography, and mass spectrometry. Herein, we report an imaging technique to visualize nascent proteins by harnessing the emerging stimulated Raman scattering (SRS) microscopy coupled with metabolic incorporation of deuterium-labeled amino acids. As a first demonstration, we imaged newly synthesized proteins in live mammalian cells with high spatial-temporal resolution without fixation or staining. Subcellular compartments with fast protein turnover in HeLa and HEK293T cells, and newly grown neurites in differentiating neuron-like N2A cells, are clearly identified via this imaging technique. Technically, incorporation of deuterium-labeled amino acids is minimally perturbative to live cells, whereas SRS imaging of exogenous carbon-deuterium bonds (C-D) in the cell-silent Raman region is highly sensitive, specific, and compatible with living systems. Moreover, coupled with label-free SRS imaging of the total proteome, our method can readily generate spatial maps of the quantitative ratio between new and total proteomes. Thus, this technique of nonlinear vibrational imaging of stable isotope incorporation will be a valuable tool to advance our understanding of the complex spatial and temporal dynamics of newly synthesized proteome in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.