ArticleJournal of biomedical optics2013
In vivo imaging of orthotopic prostate cancer with far-red gene reporter fluorescence tomography and in vivo and ex vivo validation.
Article in Journal of biomedical optics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- iRFP (near-infrared fluorescent protein) imaging of subcutaneous and deep tissue tumours in mice highlights differences between imaging platforms.Cancer cell international · 2021Article
- Non-Invasive Fluorescent Monitoring of Ovarian Cancer in an Immunocompetent Mouse Model.Cancers · 2018Article
- Near-Infrared Fluorescent Proteins: Multiplexing and Optogenetics across Scales.Trends in biotechnology · 2018Review
- Development of an inducible mouse model of iRFP713 to track recombinase activity and tumour development in vivo.Scientific reports · 2017Article
- Using Dual Fluorescence Reporting Genes to Establish an In Vivo Imaging Model of Orthotopic Lung Adenocarcinoma in Mice.Molecular imaging and biology · 2016Article
- Imaging technologies for monitoring the safety, efficacy and mechanisms of action of cell-based regenerative medicine therapies in models of kidney disease.European journal of pharmacology · 2016Review
- In Vivo Follow-up of Brain Tumor Growth via Bioluminescence Imaging and Fluorescence Tomography.International journal of molecular sciences · 2016Article
- Ultrafast excited-state dynamics and fluorescence deactivation of near-infrared fluorescent proteins engineered from bacteriophytochromes.Scientific reports · 2015Article
- Near-infrared fluorescent proteins engineered from bacterial phytochromes.Current opinion in chemical biology · 2015Review
- Emerging lymphatic imaging technologies for mouse and man.The Journal of clinical investigation · 2014Review
- In vivo lymphatic imaging of a human inflammatory breast cancer model.Journal of Cancer · 2014Article
- FLIM-FRET for Cancer Applications.Current molecular imagingArticle
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Fluorescence gene reporters have recently become available for excitation at far-red wavelengths, enabling opportunities for small animal in vivo gene reporter fluorescence tomography (GRFT). We employed multiple projections of the far-red fluorescence gene reporters IFP1.4 and iRFP, excited by a point source in transillumination geometry in order to reconstruct the location of orthotopically implanted human prostate cancer (PC3), which stably expresses the reporter. Reconstruction was performed using a linear radiative-transfer-based regularization-free tomographic method. Positron emission tomography (PET) imaging of a radiolabeled antibody-based agent that targeted epithelial cell adhesion molecule overexpressed on PC3 cells was used to confirm in vivo GRFT results. Validation of GRFT results was also conducted from ex vivo fluorescence imaging of resected prostate tumor. In addition, in mice with large primary prostate tumors, a combination of GRFT and PET showed that the radiolabeled antibody did not penetrate the tumor, consistent with known tumor transport limitations of large (∼150 kDa) molecules. These results represent the first tomography of a living animal using far-red gene reporters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.