ArticleThe Journal of general physiology2013
The mechanoelectrical response of the cytoplasmic membrane of Vibrio cholerae.
Article in The Journal of general physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Lipid-mediated gating of a miniature mechanosensitive MscS channel from Trypanosoma cruzi.Nature communications · 2025Article
- Mechanosensitive channel MscS is critical for termination of the bacterial hypoosmotic permeability response.The Journal of general physiology · 2023Article
- Cyclodextrins increase membrane tension and are universal activators of mechanosensitive channels.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Exploring the diversity of mechanosensitive channels in bacterial genomes.European biophysics journal : EBJ · 2021Article
- Charged pore-lining residues are required for normal channel kinetics in the eukaryotic mechanosensitive ion channel MSL1.Channels (Austin, Tex.) · 2020Article
- Life with Bacterial Mechanosensitive Channels, from Discovery to Physiology to Pharmacological Target.Microbiology and molecular biology reviews : MMBR · 2020Review
- An agonist of the MscL channel affects multiple bacterial species and increases membrane permeability and potency of common antibiotics.Molecular microbiology · 2019Article
- Spatiotemporal relationships defining the adaptive gating of the bacterial mechanosensitive channel MscS.European biophysics journal : EBJ · 2018Article
- Evolutionary specialization of MscCG, an MscS-like mechanosensitive channel, in amino acid transport in Corynebacterium glutamicum.Scientific reports · 2018Article
- Identification and Characterization of the Neisseria gonorrhoeae MscS-Like Mechanosensitive Channel.Infection and immunity · 2018Article
- Growth arrest and a persister state enable resistance to osmotic shock and facilitate dissemination of Vibrio cholerae.The ISME journal · 2017Article
- Tension-activated channels in the mechanism of osmotic fitness inThe Journal of general physiology · 2017Article
- MSL1 is a mechanosensitive ion channel that dissipates mitochondrial membrane potential and maintains redox homeostasis in mitochondria during abiotic stress.The Plant journal : for cell and molecular biology · 2016Article
- Bacterial Spheroplasts as a Model for Visualizing Membrane Translocation of Antimicrobial Peptides.Antimicrobial agents and chemotherapy · 2016Article
- On the mobility, membrane location and functionality of mechanosensitive channels in Escherichia coli.Scientific reports · 2016Article
- The Na+-Translocating NADH:Quinone Oxidoreductase Enhances Oxidative Stress in the Cytoplasm of Vibrio cholerae.Journal of bacteriology · 2016Article
- The evolutionary 'tinkering' of MscS-like channels: generation of structural and functional diversity.Pflugers Archiv : European journal of physiology · 2015Review
- Selectivity mechanisms in MscS-like channels: From structure to function.Channels (Austin, Tex.) · 2014Article
- MscS-like mechanosensitive channels in plants and microbes.Biochemistry · 2013Review
- Bacteria under pressure, calcium channel internalization, and why cockroaches avoid glucose-baited traps.The Journal of general physiology · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Persistence of Vibrio cholerae in waters of fluctuating salinity relies on the capacity of this facultative enteric pathogen to adapt to varying osmotic conditions. In an event of osmotic downshift, osmolytes accumulated inside the bacterium can be quickly released through tension-activated channels. With the newly established procedure of giant spheroplast preparation from V. cholerae, we performed the first patch-clamp characterization of its cytoplasmic membrane and compared tension-activated currents with those in Esherichia coli. Saturating pressure ramps revealed two waves of activation belonging to the ∼1-nS mechanosensitive channel of small conductance (MscS)-like channels and ∼3-nS mechanosensitive channel of large conductance (MscL)-like channels, with a pressure midpoint ratio p0.5MscS/p0.5MscL of 0.48. We found that MscL-like channels in V. cholerae present at a density three times higher than in E. coli, and yet, these vibrios were less tolerant to large osmotic downshocks. The Vibrio MscS-like channels exhibit characteristic inward rectification and subconductive states at depolarizing voltages; they also adapt and inactivate at subsaturating tensions and recover within 2 s upon tension release, just like E. coli MscS. Trehalose, a compatible internal osmolyte accumulated under hypertonic conditions, significantly shifts activation curves of both MscL- and MscS-like channels toward higher tensions, yet does not freely partition into the channel pore. Direct electrophysiology of V. cholerae offers new avenues for the in situ analysis of membrane components critical for osmotic survival and electrogenic transport in this pathogen.
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