ReviewDrug discovery today2013
Chemical denaturation as a tool in the formulation optimization of biologics.
Review in Drug discovery today, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Pressure Protein Denaturation Compared to Thermal and Chemical Unfolding: Analyses with Cooperative Models.The journal of physical chemistry. B · 2025Article
- Defects in the Maturation of Mitochondrial Iron-Sulfur Proteins: Biophysical Investigation of the MMDS3 Causing Gly104Cys Variant of IBA57.International journal of molecular sciences · 2024Article
- Reinforcing Protein Biochemistry: A Two-Week Experiment Studying Iron(III) Binding by the Transferrin Protein through Stoichiometric Determination, Stability Analysis, and Visualization of the Binding Site.Journal of chemical education · 2024Article
- Fluorescence-Based Protein Stability Monitoring-A Review.International journal of molecular sciences · 2024Review
- Ethanol Inhalation as a Method to Denature the Spike Protein of SARS-CoV-2.Advances in experimental medicine and biology · 2024Article
- Isothermal chemical denaturation assay for monitoring protein stability and inhibitor interactions.Scientific reports · 2023Article
- Chemical Protein Unfolding - A Simple Cooperative Model.The journal of physical chemistry. B · 2023Article
- Aggregation Time Machine: A Platform for the Prediction and Optimization of Long-Term Antibody Stability Using Short-Term Kinetic Analysis.Journal of medicinal chemistry · 2022Article
- Terahertz circular polarization sensing for protein denaturation based on a twisted dual-layer metasurface.Biomedical optics express · 2022Article
- Long-term stability predictions of therapeutic monoclonal antibodies in solution using Arrhenius-based kinetics.Scientific reports · 2021Article
- Mechanisms of sterile inflammation after intravitreal injection of antiangiogenic drugs: a narrative review.International journal of retina and vitreous · 2021Review
- Self-assembled fibrinogen-fibronectin hybrid protein nanofibers with medium-sensitive stability.RSC advances · 2021Article
- Machine learning reveals hidden stability code in protein native fluorescence.Computational and structural biotechnology journal · 2021Article
- Adapting the chemical unfolding assay for high-throughput protein screening using experimental and spectroscopic corrections.Analytical biochemistry · 2018Article
- Periplasmic Chaperones and Prolyl Isomerases.EcoSal Plus · 2018Review
- Denaturation and Aggregation of Interferon-τ in Aqueous Solution.Pharmaceutical research · 2018Article
- Temperature stability of proteins: Analysis of irreversible denaturation using isothermal calorimetry.Proteins · 2017Article
- Differential Scanning Calorimetry - A Method for Assessing the Thermal Stability and Conformation of Protein Antigen.Journal of visualized experiments : JoVE · 2017Article
- Chaperonin-Based Biolayer Interferometry To Assess the Kinetic Stability of Metastable, Aggregation-Prone Proteins.Biochemistry · 2016Article
- Conformational stability and self-association equilibrium in biologics.Drug discovery today · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Biologics have become the fastest growing segment in the pharmaceutical industry. As is the case with all proteins, biologics are susceptible to denature or to aggregate; conditions that, if present, preclude their use as pharmaceuticals. Identifying the solvent conditions that maximize their structural stability is crucial during development. Since the structural stability of a protein is susceptible to different chemical and physical conditions, the use of several complementary techniques can be expected to provide the best answers. Stability measurements that rely on temperature or chemical [urea or guanidine hydrochloride (GuHCl)] denaturation have been the preferred ones in research laboratories and together provide a thorough evaluation of protein stability. In this review, we will discuss chemical denaturation as a tool in the optimization of formulation conditions for biologics, and how chemical denaturation complements the role of thermal denaturation for this purpose.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.