ArticlePlant physiology2013
Ectopic expression of miR160 results in auxin hypersensitivity, cytokinin hyposensitivity, and inhibition of symbiotic nodule development in soybean.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
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Who cites it
88 citing papers in PubMed, 188 citations in OpenAlex.
- Small but big player: the important role of microRNAs in legume crops.Molecular genetics and genomics : MGG · 2026Review
- Systemic proteome remodeling in soybean during early nodulation: distinct responses in roots and leaves.Frontiers in plant science · 2026Article
- Hormone-modulated transcription factors orchestrating the root nodule symbiosis.Frontiers in plant science · 2026Review
- Integrated analysis of microRNAs and lncRNAs expression profiles reveals regulatory modules during adventitious shoot induction in Moringa oleifera Lam.BMC plant biology · 2024Article
- Epigenetics and plant hormone dynamics: a functional and methodological perspective.Journal of experimental botany · 2024Review
- A Germin-Like Protein GLP1 of Legumes Mediates Symbiotic Nodulation by Interacting with an Outer Membrane Protein of Rhizobia.Microbiology spectrum · 2023Article
- Integrative Transcriptome, miRNAs, Degradome, and Phytohormone Analysis ofInternational journal of molecular sciences · 2023Article
- Stu-miR827-TargetedInternational journal of molecular sciences · 2022Article
- Right time, right place: The dynamic role of hormones in rhizobial infection and nodulation of legumes.Plant communications · 2022Review
- Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops.Heredity · 2022Article
- Progress in soybean functional genomics over the past decade.Plant biotechnology journal · 2022Review
- Identification and Expression Analysis of miR160 and Their Target Genes in Cucumber.Biochemical genetics · 2022Review
- Spatiotemporal cytokinin response imaging and ISOPENTENYLTRANSFERASE 3 function in Medicago nodule development.Plant physiology · 2022Article
- MicroRNAs Are Involved in Regulating Plant Development and Stress Response through Fine-Tuning of TIR1/AFB-Dependent Auxin Signaling.International journal of molecular sciences · 2022Review
- A Multi-Level Iterative Bi-Clustering Method for Discovering miRNA Co-regulation Network of Abiotic Stress Tolerance in Soybeans.Frontiers in plant science · 2022Article
- miR160: An Indispensable Regulator in Plant.Frontiers in plant science · 2022Review
- Roles of non-coding RNAs in the hormonal and nutritional regulation in nodulation and nitrogen fixation.Frontiers in plant science · 2022Review
- Roles of microRNAs in abiotic stress response and characteristics regulation of plant.Frontiers in plant science · 2022Review
- CRISPR/Cas9 mutants of tomato MICRORNA164 genes uncover their functional specialization in development.Plant physiology · 2021Article
- GmPIN-dependent polar auxin transport is involved in soybean nodule development.The Plant cell · 2021Article
28 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Symbiotic root nodules in leguminous plants result from interaction between the plant and nitrogen-fixing rhizobia bacteria. There are two major types of legume nodules, determinate and indeterminate. Determinate nodules do not have a persistent meristem, while indeterminate nodules have a persistent meristem. Auxin is thought to play a role in the development of both these types of nodules. However, inhibition of rootward auxin transport at the site of nodule initiation is crucial for the development of indeterminate nodules but not determinate nodules. Using the synthetic auxin-responsive DR5 promoter in soybean (Glycine max), we show that there is relatively low auxin activity during determinate nodule initiation and that it is restricted to the nodule periphery subsequently during development. To examine if and what role auxin plays in determinate nodule development, we generated soybean composite plants with altered sensitivity to auxin. We overexpressed microRNA393 to silence the auxin receptor gene family, and these roots were hyposensitive to auxin. These roots nodulated normally, suggesting that only minimal/reduced auxin signaling is required for determinate nodule development. We overexpressed microRNA160 to silence a set of repressor auxin response factor transcription factors, and these roots were hypersensitive to auxin. These roots were not impaired in epidermal responses to rhizobia but had significantly reduced nodule primordium formation, suggesting that auxin hypersensitivity inhibits nodule development. These roots were also hyposensitive to cytokinin and had attenuated expression of key nodulation-associated transcription factors known to be regulated by cytokinin. We propose a regulatory feedback loop involving auxin and cytokinin during nodulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.