ArticleJournal of molecular biology2013
Resurrection of an Urbilaterian U1A/U2B″/SNF protein.
Article in Journal of molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- SNRPB2 facilitates esophageal squamous cell carcinoma oncogenesis and progression via E2F4 stabilization.Frontiers in immunology · 2025Article
- Integrating Phylogenetics With Intron Positions Illuminates the Origin of the Complex Spliceosome.Molecular biology and evolution · 2023Article
- Phylogenetic analysis and stress response of the plant U2 small nuclear ribonucleoprotein B″ gene family.BMC genomics · 2022Article
- Phylogeny and conservation of plant U2A/U2A', a core splicing component in U2 spliceosomal complex.Planta · 2021Article
- Molecular principles underlying dual RNA specificity in the Drosophila SNF protein.Nature communications · 2018Article
- Robustness of Reconstructed Ancestral Protein Functions to Statistical Uncertainty.Molecular biology and evolution · 2017Article
- Resurrecting ancestral structural dynamics of an antiviral immune receptor: adaptive binding pocket reorganization repeatedly shifts RNA preference.BMC evolutionary biology · 2016Article
- Co-evolution of SNF spliceosomal proteins with their RNA targets in trans-splicing nematodes.Genetica · 2016Article
- Urb-RIP - An Adaptable and Efficient Approach for Immunoprecipitation of RNAs and Associated RNAs/Proteins.PloS one · 2016Article
- Climbing the vertebrate branch of U1A/U2B″ protein evolution.RNA (New York, N.Y.) · 2014Article
- A compare-and-contrast NMR dynamics study of two related RRMs: U1A and SNF.Biophysical journal · 2014Article
- Binding affinity and cooperativity control U2B″/snRNA/U2A' RNP formation.Biochemistry · 2014Article
- Linkage and allostery in snRNP protein/RNA complexes.Biochemistry · 2014Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The U1A/U2B″/SNF family of proteins found in the U1 and U2 spliceosomal small nuclear ribonucleoproteins is highly conserved. In spite of the high degree of sequence and structural conservation, modern members of this protein family have unique RNA binding properties. These differences have necessarily resulted from evolutionary processes, and therefore, we reconstructed the protein phylogeny in order to understand how and when divergence occurred and how protein function has been modulated. Contrary to the conventional understanding of an ancient human U1A/U2B″ gene duplication, we show that the last common ancestor of bilaterians contained a single ancestral protein (URB). The gene for URB was synthesized, the protein was overexpressed and purified, and we assessed RNA binding to modern snRNA sequences. We find that URB binds human and Drosophila U1 snRNA SLII and U2 snRNA SLIV with higher affinity than do modern homologs, suggesting that both Drosophila SNF and human U1A/U2B″ have evolved into weaker binders of one RNA or both RNAs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.