ArticleAnalytical methods : advancing methods and applications2013
Quantitation of dopamine, serotonin and adenosine content in a tissue punch from a brain slice using capillary electrophoresis with fast-scan cyclic voltammetry detection.
Article in Analytical methods : advancing methods and applications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 64 citations in OpenAlex.
- A genetic model for development, physiology and behavior of catecholamine-deficient zebrafish larvae.Communications biology · 2026Article
- Bioanalytical HPLC-UV Determination of Dopamine in Plasma and Mouse Brain Homogenate with Greenness, Whiteness, and Blueness Assessment.Molecules (Basel, Switzerland) · 2026Article
- Capillary Electrophoresis With Amperometric Detection for Neurotransmitter Analysis: Principles, Electrode Materials, Methodologies, and Applications.Electrophoresis · 2026Review
- Scalable, flexible carbon fiber electrode thread arrays for three-dimensional probing of neurochemical activity in deep brain structures of rodents.Biosensors & bioelectronics · 2023Article
- Assisted Reductive Amination for Quantitation of Tryptophan, 5-Hydroxytryptophan, and Serotonin by Ultraperformance Liquid Chromatography Coupled with Tandem Mass Spectrometry.Molecules (Basel, Switzerland) · 2023Article
- Activated Glassy Carbon Electrode as an Electrochemical Sensing Platform for the Determination of 4-Nitrophenol and Dopamine in Real Samples.ACS omega · 2022Article
- Porous Carbon Nanofiber-Modified Carbon Fiber Microelectrodes for Dopamine Detection.ACS applied nano materials · 2022Article
- Measurement of natural variation of neurotransmitter tissue content in red harvester ant brains among different colonies.Analytical and bioanalytical chemistry · 2020Article
- Hybrid Graphene/Conducting Polymer Strip Sensors for Sensitive and Selective Electrochemical Detection of Serotonin.ACS omega · 2019Article
- New optical methods for detecting monoamine neuromodulators.Current opinion in biomedical engineering · 2019Article
- Nanodiamond Coating Improves the Sensitivity and Antifouling Properties of Carbon Fiber Microelectrodes.ACS sensors · 2019Article
- Polymer Modified Carbon Fiber-Microelectrodes and Waveform Modifications Enhance Neurotransmitter Metabolite Detection.Analytical methods : advancing methods and applications · 2019Article
- Colorimetric adenosine aptasensor based on DNA cycling amplification and salt-induced aggregation of gold nanoparticles.Mikrochimica acta · 2018Article
- Microchip electrophoresis with electrochemical detection for the determination of analytes in the dopamine metabolic pathway.Electrophoresis · 2015Article
- Analysis of neurotransmitter tissue content of Drosophila melanogaster in different life stages.ACS chemical neuroscience · 2015Article
- Future approaches to therapeutic hypothermia: a symposium report.Temperature (Austin, Tex.)Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Methods to determine neurochemical concentrations in small samples of tissue are needed to map interactions among neurotransmitters. In particular, correlating physiological measurements of neurotransmitter release and the tissue content in a small region would be valuable. HPLC is the standard method for tissue content analysis but it requires microliter samples and the detector often varies by the class of compound being quantified; thus detecting molecules from different classes can be difficult. In this paper, we develop capillary electrophoresis with fast-scan cyclic voltammetry detection (CE-FSCV) for analysis of dopamine, serotonin, and adenosine content in tissue punches from rat brain slices. Using field-amplified sample stacking, the limit of detection was 5 nM for dopamine, 10 nM for serotonin, and 50 nM for adenosine. Neurotransmitters could be measured from a tissue punch as small as 7 µg (7 nL) of tissue, three orders of magnitude smaller than a typical HPLC sample. Tissue content analysis of punches in successive slices through the striatum revealed higher dopamine but lower adenosine content in the anterior striatum. Stimulated dopamine release was measured in a brain slice, then a tissue punch collected from the recording region. Dopamine content and release had a correlation coefficient of 0.71, which indicates much of the variance in stimulated release is due to variance in tissue content. CE-FSCV should facilitate measurements of tissue content in nanoliter samples, leading to a better understanding of how diseases or drugs affect dopamine, serotonin, and adenosine content.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.