ArticleJournal of bacteriology2013
The Clostridium difficile exosporium cysteine (CdeC)-rich protein is required for exosporium morphogenesis and coat assembly.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- Coupling Far-Western Blotting with Peptide Microarrays Reveals Novel E-Cadherin Spore-Surface Ligands inJournal of proteome research · 2026Article
- Development and Evaluation of Exosporium-Anchored Bioluminescent and Fluorescent Reporters for TrackingACS synthetic biology · 2026Article
- Preparation of Spores with the 70:30 Method.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Article
- Spores of Clostridioides difficile are toxin delivery vehicles.Communications biology · 2024Article
- RelQ-mediated alarmone signalling regulates growth, stress-induced biofilm formation and spore accumulation inMicrobiology (Reading, England) · 2024Article
- RelQ-mediated alarmone signaling regulates growth, sporulation, and stress-induced biofilm formation inbioRxiv : the preprint server for biology · 2024Article
- Clostridioides difficile Sporulation.Advances in experimental medicine and biology · 2024Review
- Immunization Strategies Against Clostridioides difficile.Advances in experimental medicine and biology · 2024Review
- A sporulation signature protease is required for assembly of the spore surface layers, germination and host colonization in Clostridioides difficile.PLoS pathogens · 2023Article
- Review
- Survival of Clostridioides difficile spores in thermal and chemo-thermal laundering processes and influence of the exosporium on their adherence to cotton bed sheets.Letters in applied microbiology · 2022Article
- Potential Risk of Botulinum Neurotoxin -producing Clostridia Occurrence in Canned Fish.Journal of veterinary research · 2022Article
- Role of the Spore Coat ProteinsMicroorganisms · 2022Article
- Practical observations on the use of fluorescent reporter systems in Clostridioides difficile.Antonie van Leeuwenhoek · 2022Article
- Immunoinformatics Approach Toward the Introduction of a Novel Multi-Epitope Vaccine AgainstFrontiers in immunology · 2022Article
- Opportunities for Nanomedicine inAntibiotics (Basel, Switzerland) · 2021Review
- Entry of spores into intestinal epithelial cells contributes to recurrence of Clostridioides difficile infection.Nature communications · 2021Article
- Biofilms harbour Clostridioides difficile, serving as a reservoir for recurrent infection.NPJ biofilms and microbiomes · 2021Article
- Learning from Nature: Bacterial Spores as a Target for Current Technologies in Medicine (Review).Sovremennye tekhnologii v meditsine · 2021Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clostridium difficile is an important nosocomial pathogen that has become a major cause of antibiotic-associated diarrhea. There is a general consensus that C. difficile spores play an important role in C. difficile pathogenesis, contributing to infection, persistence, and transmission. Evidence has demonstrated that C. difficile spores have an outermost layer, termed the exosporium, that plays some role in adherence to intestinal epithelial cells. Recently, the protein encoded by CD1067 was shown to be present in trypsin-exosporium extracts of C. difficile 630 spores. In this study, we renamed the CD1067 protein Clostridium difficile exosporium cysteine-rich protein (CdeC) and characterized its role in the structure and properties of C. difficile spores. CdeC is expressed under sporulation conditions and localizes to the C. difficile spore. Through the construction of an ΔcdeC isogenic knockout mutant derivative of C. difficile strain R20291, we demonstrated that (i) the distinctive nap layer is largely missing in ΔcdeC spores; (ii) CdeC is localized in the exosporium-like layer and is accessible to IgGs; (iii) ΔcdeC spores were more sensitive to lysozyme, ethanol, and heat treatment than wild-type spores; and (iv) despite the almost complete absence of the exosporium layer, ΔcdeC spores adhered at higher levels than wild-type spores to intestinal epithelium cell lines (i.e., HT-29 and Caco-2 cells). Collectively, these results indicate that CdeC is essential for exosporium morphogenesis and the correct assembly of the spore coat of C. difficile.
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