ReviewPlant molecular biology2013
The yeast two-hybrid and related methods as powerful tools to study plant cell signalling.
Review in Plant molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Artificial intelligence-driven multi-omics integration for plant enhancement: advances, challenges, and future perspectives.Functional & integrative genomics · 2026Review
- Antibody-oligonucleotide conjugates for spatial proteomics: principles, applications, and challenges.Acta biochimica et biophysica Sinica · 2025Article
- The Constrained Disorder Principle: A Paradigm Shift for Accurate Interactome Mapping and Information Analysis in Complex Biological Systems.Bioengineering (Basel, Switzerland) · 2025Review
- Cellular view of metabolism: metabolic biomolecular condensates.The New phytologist · 2025Review
- Identification and characterization of TCP transcription factor GmTCP670 associated with soybean development.Scientific reports · 2025Article
- A core Plasmopara viticola effector attenuates the DNA-binding activity of bZIP transcription factor to compromise plant immunity.The Plant journal : for cell and molecular biology · 2025Article
- Workflow to Select Functional Promoter DNA Baits and Screen Arrayed Gene Libraries in Yeast.Current protocols · 2024Article
- Screening of Tnfaip1-Interacting Proteins in Zebrafish Embryonic cDNA Libraries Using a Yeast Two-Hybrid System.Current issues in molecular biology · 2023Article
- Yeast Heterologous Expression Systems for the Study of Plant Membrane Proteins.International journal of molecular sciences · 2023Review
- Potential application of TurboID-based proximity labeling in studying the protein interaction network in plant response to abiotic stress.Frontiers in plant science · 2022Review
- A LexA-based yeast two-hybrid system for studying light-switchable interactions of phytochromes with their interacting partners.aBIOTECH · 2021Article
- Novel assays to monitor gene expression and protein-protein interactions in rice using the bioluminescent protein, NanoLuc.Plant biotechnology (Tokyo, Japan) · 2021Article
- Interactomes: Experimental and In Silico Approaches.Advances in experimental medicine and biology · 2021Article
- Protein Complex Identification and quantitative complexome by CN-PAGE.Scientific reports · 2019Article
- The Methods Employed in Mass Spectrometric Analysis of Posttranslational Modifications (PTMs) and Protein-Protein Interactions (PPIs).Advances in experimental medicine and biology · 2019Review
- Reconstruction and Application of Protein-Protein Interaction Network.International journal of molecular sciences · 2016Review
- Fundamentals of protein interaction network mapping.Molecular systems biology · 2015Review
- Peptide aptamers: development and applications.Current topics in medicinal chemistry · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One basic property of proteins is their ability to specifically target and form non-covalent complexes with other proteins. Such protein-protein interactions play key roles in all biological processes, extending from the formation of cellular macromolecular structures and enzymatic complexes to the regulation of signal transduction pathways. Identifying and characterizing protein interactions and entire interaction networks (interactomes) is therefore prerequisite to understand these processes on a molecular and biophysical level. Since its original description in 1989, the yeast two-hybrid system has been extensively used to identify protein-protein interactions from many different organisms, thus providing a convenient mean to both screen for proteins that interact with a protein of interest and to characterize the known interaction between two proteins. In these years the technique has improved to overcome the limitations of the original assay, and many efforts have been made to scale up the technique and to adapt it to large scale studies. In addition, variations have been introduced to enlarge the range of proteins and interactors that can be assayed by hybrid-based approaches. Several groups studying molecular mechanisms that underlie plant cell signal transduction pathways have successfully used the yeast two-hybrid system or related methods. In this review we provide a brief description of the technology, attempt to point out some of the pitfalls and benefits of the different systems that can be employed, and mention some of the areas, within the plant cell signalling field, where hybrid-based interaction assays have been particularly informative.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.