ArticleApplied and environmental microbiology2013
Novel, oxygen-insensitive group 5 [NiFe]-hydrogenase in Ralstonia eutropha.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 94 citations in OpenAlex.
- Search for the role of a Huc-type [NiFe]-hydrogenase of the soil thermophileFrontiers in microbiology · 2026Article
- Hydrogen Oxidation Benefits Alphaproteobacterial Methanotrophs Under Severe Methane Limitation.Environmental microbiology · 2025Article
- Physiology-informed use of Cupriavidus necator in biomanufacturing: a review of advances and challenges.Microbial cell factories · 2025Review
- The energy metabolism ofApplied and environmental microbiology · 2024Article
- Outer-sphere effects on the OChemical science · 2024Review
- Improved preculture management for Cupriavidus necator cultivations.Biotechnology letters · 2023Article
- Developing high-affinity, oxygen-insensitive [NiFe]-hydrogenases as biocatalysts for energy conversion.Biochemical Society transactions · 2023Review
- Photosystem II for photoelectrochemical hydrogen production.Biophysical reviews · 2023Review
- L-amino acids affect the hydrogenase activity and growth of Ralstonia eutropha H16.AMB Express · 2023Article
- Article
- Hydrogenase and Nitrogenase: Key Catalysts in Biohydrogen Production.Molecules (Basel, Switzerland) · 2023Review
- Novel concepts and engineering strategies for heterologous expression of efficient hydrogenases in photosynthetic microorganisms.Frontiers in microbiology · 2023Review
- Engineering a Rhodopsin-Based Photo-Electrosynthetic System in Bacteria for COACS synthetic biology · 2022Article
- Microbial oxidation of atmospheric trace gases.Nature reviews. Microbiology · 2022Review
- Multiple energy sources and metabolic strategies sustain microbial diversity in Antarctic desert soils.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Verrucomicrobial methanotrophs: ecophysiology of metabolically versatile acidophiles.FEMS microbiology reviews · 2021Review
- A widely distributed hydrogenase oxidises atmospheric HThe ISME journal · 2020Article
- The thermoacidophilic methanotroph Methylacidiphilum fumariolicum SolV oxidizes subatmospheric HThe ISME journal · 2020Article
- Two uptake hydrogenases differentially interact with the aerobic respiratory chain during mycobacterial growth and persistence.The Journal of biological chemistry · 2019Article
- Growth of the facultative chemolithoautotroph Ralstonia eutropha on organic waste materials: growth characteristics, redox regulation and hydrogenase activity.Microbial cell factories · 2019Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently, a novel group of [NiFe]-hydrogenases has been defined that appear to have a great impact in the global hydrogen cycle. This so-called group 5 [NiFe]-hydrogenase is widespread in soil-living actinobacteria and can oxidize molecular hydrogen at atmospheric levels, which suggests a high affinity of the enzyme toward H2. Here, we provide a biochemical characterization of a group 5 hydrogenase from the betaproteobacterium Ralstonia eutropha H16. The hydrogenase was designated an actinobacterial hydrogenase (AH) and is catalytically active, as shown by the in vivo H2 uptake and by activity staining in native gels. However, the enzyme does not sustain autotrophic growth on H2. The AH was purified to homogeneity by affinity chromatography and consists of two subunits with molecular masses of 65 and 37 kDa. Among the electron acceptors tested, nitroblue tetrazolium chloride was reduced by the AH at highest rates. At 30°C and pH 8, the specific activity of the enzyme was 0.3 μmol of H2 per min and mg of protein. However, an unexpectedly high Michaelis constant (Km) for H2 of 3.6 ± 0.5 μM was determined, which is in contrast to the previously proposed low Km of group 5 hydrogenases and makes atmospheric H2 uptake by R. eutropha most unlikely. Amperometric activity measurements revealed that the AH maintains full H2 oxidation activity even at atmospheric oxygen concentrations, showing that the enzyme is insensitive toward O2.
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