ArticleActa crystallographica. Section D, Biological crystallography2013
Robust structural analysis of native biological macromolecules from multi-crystal anomalous diffraction data.
Article in Acta crystallographica. Section D, Biological crystallography, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
60 citing papers in PubMed.
- LEGO®-inspired electrically-actuated microfluidics for on-chip protein crystallization andLab on a chip · 2026Article
- Evolution of macromolecular crystallography beamlines at the Swiss Light Source and SwissFEL.Journal of synchrotron radiation · 2025Article
- Nickel binding to c-Src SH3 domain facilitates crystallization.bioRxiv : the preprint server for biology · 2025Article
- Scalable fabrication of an array-type fixed-target device for automated room temperature X-ray protein crystallography.Scientific reports · 2025Article
- Useful experimental aspects of small-wedge synchrotron crystallography for accurate structure analysis of protein molecules.Acta crystallographica. Section D, Structural biology · 2025Article
- Nickel Binding to the c-Src SH3 Domain Facilitates Crystallization.Protein and peptide letters · 2025Article
- Structure of the RNA-dependent RNA polymerase P2 from the cystovirus φ8.Scientific reports · 2024Article
- Utilizing anomalous signals for element identification in macromolecular crystallography.Acta crystallographica. Section D, Structural biology · 2024Article
- X-Ray Crystallography for Macromolecular Complexes.Advances in experimental medicine and biology · 2024Article
- Facing the phase problem.IUCrJ · 2023Article
- Polymer-based microfluidic device for on-chip counter-diffusive crystallization andLab on a chip · 2023Article
- Article
- Serial crystallography with multi-stage merging of thousands of images.Acta crystallographica. Section F, Structural biology communications · 2022Article
- ID23-2: an automated and high-performance microfocus beamline for macromolecular crystallography at the ESRF.Journal of synchrotron radiation · 2022Article
- Guidelines for de novo phasing using multiple small-wedge data collection.Journal of synchrotron radiation · 2021Article
- High-throughput in situ experimental phasing.Acta crystallographica. Section D, Structural biology · 2020Article
- Article
- Bayesian machine learning improves single-wavelength anomalous diffraction phasing.Acta crystallographica. Section A, Foundations and advances · 2019Article
- Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Structure determinations for biological macromolecules that have no known structural antecedents typically involve the incorporation of heavier atoms than those found natively in biological molecules. Currently, selenomethionyl proteins analyzed using single- or multi-wavelength anomalous diffraction (SAD or MAD) data predominate for such de novo analyses. Naturally occurring metal ions such as zinc or iron often suffice in MAD or SAD experiments, and sulfur SAD has been an option since it was first demonstrated using crambin 30 years ago; however, SAD analyses of structures containing only light atoms (Zmax ≤ 20) have not been common. Here, robust procedures for enhancing the signal to noise in measurements of anomalous diffraction by combining data collected from several crystals at a lower than usual X-ray energy are described. This multi-crystal native SAD method was applied in five structure determinations, using between five and 13 crystals to determine substructures of between four and 52 anomalous scatterers (Z ≤ 20) and then the full structures ranging from 127 to 1200 ordered residues per asymmetric unit at resolutions from 2.3 to 2.8 Å. Tests were devised to assure that all of the crystals used were statistically equivalent. Elemental identities for Ca, Cl, S, P and Mg were proven by f'' scattering-factor refinements. The procedures are robust, indicating that truly routine structure determination of typical native macromolecules is realised. Synchrotron beamlines that are optimized for low-energy X-ray diffraction measurements will facilitate such direct structural analysis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.