ArticleNaunyn-Schmiedeberg's archives of pharmacology2013
Lack of specificity shown by P2Y6 receptor antibodies.
Article in Naunyn-Schmiedeberg's archives of pharmacology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 36 citations in OpenAlex.
- Microglial P2YNeuron · 2024Article
- Open Science 2.0: Towards a truly collaborative research ecosystem.PLoS biology · 2023Article
- Potential determinants of low circulating glucagon-like peptide 2 concentrations in Zambian children with non-responsive stunting.Experimental physiology · 2023Article
- Amplified CaInternational journal of cancer · 2022Article
- Review
- Retinoid acid induced 16 deficiency aggravates colitis and colitis-associated tumorigenesis in mice.Cell death & disease · 2019Article
- Reviewing the role of P2Y receptors in specific gastrointestinal cancers.Purinergic signalling · 2019Article
- No answer to the lack of specificity: mouse monoclonal antibody targeting the angiotensin II type 1 receptor ATNaunyn-Schmiedeberg's archives of pharmacology · 2018Article
- P2YScientific reports · 2017Article
- Generation and Characterization of Specific Antibodies to the Murine and Human Ectonucleotidase NTPDase8.Frontiers in pharmacology · 2017Article
- Altered purinergic receptor-Ca²⁺ signaling associated with hypoxia-induced epithelial-mesenchymal transition in breast cancer cells.Molecular oncology · 2016Article
- Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS).PloS one · 2015Article
- The PRKAA1/AMPKα1 pathway triggers autophagy during CSF1-induced human monocyte differentiation and is a potential target in CMML.Autophagy · 2015Article
- P2Y receptors in Alzheimer's disease.Biology of the cell · 2015Review
- P2Y₆ receptor inhibition perturbs CCL2-evoked signalling in human monocytic and peripheral blood mononuclear cells.Journal of cell science · 2014Article
- Rat β₃-adrenoceptor protein expression: antibody validation and distribution in rat gastrointestinal and urogenital tissues.Naunyn-Schmiedeberg's archives of pharmacology · 2014Article
- Antibodies for immunolabeling by light and electron microscopy: not for the faint hearted.Histochemistry and cell biology · 2014Review
- Microglia P2Y₆ receptors mediate nitric oxide release and astrocyte apoptosis.Journal of neuroinflammation · 2014Article
- Activation of P2Y6 receptors increases the voiding frequency in anaesthetized rats by releasing ATP from the bladder urothelium.British journal of pharmacology · 2014Article
- Human urothelial cell lines as potential models for studying cannabinoid and excitatory receptor interactions in the urinary bladder.Naunyn-Schmiedeberg's archives of pharmacology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
P2Y6 receptor in bladder smooth muscle responds to UDP by increasing muscle tone and augmenting bladder contractions. The exact cellular location of the receptor is however unknown. Three commercially available antibodies to P2Y6 receptor gave clean bands on Western blot which were eliminated by specific peptide competition. Two of the three also immunostained bladder smooth muscle cells while leaving adjacent interstitial cells of Cajal unstained. However, attempts to validate the specificity of these antibodies by performing the same assays on bladders from P2Y6 knockout mice were unsuccessful. In Western blots, all three antibodies bound similar proteins in both wild type and P2Y6 knockout tissue. Immunostaining of knockout tissue sections also showed no difference in staining patterns or intensity. We conclude that rigorous controls are required when using commercial reagents to this G-protein coupled receptor and perhaps to other members of the P2Y receptor family.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.