ArticleThe Journal of biological chemistry2013
Efficient purification and reconstitution of ATP binding cassette transporter B6 (ABCB6) for functional and structural studies.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- TargetingZhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2026Article
- Conformational Plasticity of LptC Regulates Lipopolysaccharide Transport by the LptBJournal of the American Chemical Society · 2025Article
- A Structural Bioinformatics-Guided Study of Adenosine Triphosphate-Binding Cassette (ABC) Transporters and Their Substrates.Membranes · 2025Article
- Identification of two novel heterodimeric ABC transporters in melanoma: ABCB5β/B6 and ABCB5β/B9.The Journal of biological chemistry · 2024Article
- W546 stacking disruption traps the human porphyrin transporter ABCB6 in an outward-facing transient state.Communications biology · 2023Article
- Structural Insights into Porphyrin Recognition by the Human ATP-Binding Cassette Transporter ABCB6.Molecules and cells · 2022Article
- Regulation of Heme Synthesis by Mitochondrial Homeostasis Proteins.Frontiers in cell and developmental biology · 2022Review
- Molecular insights into the human ABCB6 transporter.Cell discovery · 2021Article
- Key transporters leading to specific protoporphyrin IX accumulation in cancer cell following administration of aminolevulinic acid in photodynamic therapy/diagnosis.International journal of clinical oncology · 2021Review
- The human ABCB6 protein is the functional homologue of HMT-1 proteins mediating cadmium detoxification.Cellular and molecular life sciences : CMLS · 2019Article
- Erythrocytes lacking the Langereis blood group protein ABCB6 are resistant to the malaria parasiteCommunications biology · 2018Article
- ABCB6, an ABC Transporter Impacting Drug Response and Disease.The AAPS journal · 2017Review
- A heme-binding domain controls regulation of ATP-dependent potassium channels.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Role of the N-terminal transmembrane domain in the endo-lysosomal targeting and function of the human ABCB6 protein.The Biochemical journal · 2015Article
- Functional coupling of ATP-binding cassette transporter Abcb6 to cytochrome P450 expression and activity in liver.The Journal of biological chemistry · 2015Article
- TMEM14C is required for erythroid mitochondrial heme metabolism.The Journal of clinical investigation · 2014Article
- Tissue-specific transcript profiling for ABC transporters in the sequestering larvae of the phytophagous leaf beetle Chrysomela populi.PloS one · 2014Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
The mitochondrial ATP binding cassette transporter ABCB6 has been associated with a broad range of physiological functions, including growth and development, therapy-related drug resistance, and the new blood group system Langereis. ABCB6 has been proposed to regulate heme synthesis by shuttling coproporphyrinogen III from the cytoplasm into the mitochondria. However, direct functional information of the transport complex is not known. To understand the role of ABCB6 in mitochondrial transport, we developed an in vitro system with pure and active protein. ABCB6 overexpressed in HEK293 cells was solubilized from mitochondrial membranes and purified to homogeneity. Purified ABCB6 showed a high binding affinity for MgATP (Kd = 0.18 μM) and an ATPase activity with a Km of 0.99 mM. Reconstitution of ABCB6 into liposomes allowed biochemical characterization of the ATPase including (i) substrate-stimulated ATPase activity, (ii) transport kinetics of its proposed endogenous substrate coproporphyrinogen III, and (iii) transport kinetics of substrates identified using a high throughput screening assay. Mutagenesis of the conserved lysine to alanine (K629A) in the Walker A motif abolished ATP hydrolysis and substrate transport. These results suggest a direct interaction between mitochondrial ABCB6 and its transport substrates that is critical for the activity of the transporter. Furthermore, the simple immunoaffinity purification of ABCB6 to near homogeneity and efficient reconstitution of ABCB6 into liposomes might provide the basis for future studies on the structure/function of ABCB6.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.