ArticleNature neuroscience2013
SeeDB: a simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction.
Article in Nature neuroscience, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 499 papers.
What it found
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Who cites it
499 citing papers in PubMed, 895 citations in OpenAlex.
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- A uniform tissue-clearing framework and mesoSPIM-ultra enable cm-scale single-neuron tracing.bioRxiv : the preprint server for biology · 2026Article
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- Loss of type III collagen has limited effect on tendon healing in young adult mice.Matrix biology : journal of the International Society for Matrix Biology · 2026Article
- SynClear: A one-step synchronous clearing and labeling strategy for multiscale 3D brain mapping.Materials today. Bio · 2026Article
- NucVerse3D: generalizable 3D nuclear instance segmentation across heterogeneous microscopy modalities.Scientific reports · 2026Article
- 3D imaging with enhanced transparency, signal-to-background ratios, and antigen detection using HyPer-3D.Cell reports methods · 2026Article
- Heterogeneity of White Matter Structure in the Human Brain.Research square · 2026Article
- Heterogeneity of white-matter organization in the human brain.bioRxiv : the preprint server for biology · 2026Article
- Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo.Nature methods · 2026Article
- Live imaging of neuronal dynamics in transparent mouse brains.Nature methods · 2026Article
- ClearScope: A Fully Integrated Light-Sheet Theta Microscope for Sub-Micron-Resolution Imaging Without Lateral Size Constraints.Journal of imaging · 2026Article
- Article
- Advances in Optical Tissue Clearing for Three-Dimensional Ocular Imaging and Analysis.Journal of ophthalmology · 2026Review
- Current progress of labeling strategies in tissue clearing for large-scale biological visualization.Frontiers in cell and developmental biology · 2026Review
- High-Throughput Volumetric Mapping Facilitated by Active Tissue SHRINK.Small methods · 2026Article
- Innovative Technologies for Kidney Research: Three-Dimensional Imaging and Quantification.Seminars in nephrology · 2026Review
439 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report a water-based optical clearing agent, SeeDB, which clears fixed brain samples in a few days without quenching many types of fluorescent dyes, including fluorescent proteins and lipophilic neuronal tracers. Our method maintained a constant sample volume during the clearing procedure, an important factor for keeping cellular morphology intact, and facilitated the quantitative reconstruction of neuronal circuits. Combined with two-photon microscopy and an optimized objective lens, we were able to image the mouse brain from the dorsal to the ventral side. We used SeeDB to describe the near-complete wiring diagram of sister mitral cells associated with a common glomerulus in the mouse olfactory bulb. We found the diversity of dendrite wiring patterns among sister mitral cells, and our results provide an anatomical basis for non-redundant odor coding by these neurons. Our simple and efficient method is useful for imaging intact morphological architecture at large scales in both the adult and developing brains.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.