ArticleThe EMBO journal2013
Ultra-deep profiling of alternatively spliced Drosophila Dscam isoforms by circularization-assisted multi-segment sequencing.
Article in The EMBO journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 73 citations in OpenAlex.
- Regulatory paradigm of Dscam1 stochastic alternative splicing through conserved long-range RNA structures.Nucleic acids research · 2026Article
- The modern expansion of Dscam1 isoform diversity in Drosophila is linked to fitness and immunity.PLoS biology · 2025Article
- The Role of Alternative Splicing in Marine-Freshwater Divergence in Threespine Stickleback.Genome biology and evolution · 2025Article
- Exploring perspectives of Dscam for cognitive deficits: a review of multifunction for regulating neural wiring in homeostasis.Frontiers in molecular neuroscience · 2025Review
- Article
- Adjacent Neuronal Fascicle Guides Motoneuron 24 Dendritic Branching and Axonal Routing Decisions through Dscam1 Signaling.bioRxiv : the preprint server for biology · 2024Article
- A systematic CRISPR screen reveals redundant and specific roles for Dscam1 isoform diversity in neuronal wiring.PLoS biology · 2023Article
- Trans-splicing facilitated by RNA pairing greatly expands sDscam isoform diversity but not homophilic binding specificity.Science advances · 2022Article
- Complex regulation of Gephyrin splicing is a determinant of inhibitory postsynaptic diversity.Nature communications · 2022Article
- Hidden RNA pairings counteract the "first-come, first-served" splicing principle to drive stochastic choice inScience advances · 2022Article
- Chelicerata sDscam isoforms combine homophilic specificities to define unique cell recognition.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- RNA secondary structures inRNA (New York, N.Y.) · 2020Article
- Long-read sequencing reveals the complex splicing profile of the psychiatric risk gene CACNA1C in human brain.Molecular psychiatry · 2020Article
- Acute thiamethoxam toxicity in honeybees is not enhanced by common fungicide and herbicide and lacks stress-induced changes in mRNA splicing.Scientific reports · 2019Article
- Srrm234, but not canonical SR and hnRNP proteins, drive inclusion ofRNA (New York, N.Y.) · 2019Article
- A Sam68-dependent alternative splicing program shapes postsynaptic protein complexes.The European journal of neuroscience · 2019Article
- Nuclear import of the DSCAM-cytoplasmic domain drives signaling capable of inhibiting synapse formation.The EMBO journal · 2019Article
- Plasmid-based gap-repair recombineered transgenes reveal a central role for introns in mutually exclusive alternative splicing in Down Syndrome Cell Adhesion Molecule exon 4.Nucleic acids research · 2019Article
- Revisiting Dscam diversity: lessons from clustered protocadherins.Cellular and molecular life sciences : CMLS · 2019Review
- Regulation of Neuronal Differentiation, Function, and Plasticity by Alternative Splicing.Annual review of cell and developmental biology · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Drosophila melanogaster gene Dscam (Down syndrome cell adhesion molecule) can generate thousands of different ectodomains via mutual exclusive splicing of three large exon clusters. The isoform diversity plays a profound role in both neuronal wiring and pathogen recognition. However, the isoform expression pattern at the global level remained unexplored. Here, we developed a novel method that allows for direct quantification of the alternatively spliced exon combinations from over hundreds of millions of Dscam transcripts in one sequencing run. With unprecedented sequencing depth, we detected a total of 18,496 isoforms, out of 19,008 theoretically possible combinations. Importantly, we demonstrated that alternative splicing between different clusters is independent. Moreover, the isoforms were expressed across a broad dynamic range, with significant bias in cell/tissue and developmental stage-specific patterns. Hitherto underappreciated, such bias can dramatically reduce the ability of neurons to display unique surface receptor codes. Therefore, the seemingly excessive diversity encoded in the Dscam locus might nevertheless be essential for a robust self and non-self discrimination in neurons.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.