ArticleMethods (San Diego, Calif.)2013
Fluorescent activated cell sorting (FACS) combined with gene expression microarrays for transcription enrichment profiling of zebrafish lateral line cells.
Article in Methods (San Diego, Calif.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- miR-133a-3p and miR-338-3p shape neural crest derivatives in zebrafish.Life science alliance · 2026Article
- A novel fluorescence-activated cell sorting (FACS)-based screening identified ATG14, the gene required for pexophagy in the methylotrophic yeast.FEMS yeast research · 2024Article
- Proximity Labeling in Plants.Annual review of plant biology · 2023Review
- Article
- Zebrafish Embryos and Larvae as Alternative Animal Models for Toxicity Testing.International journal of molecular sciences · 2021Review
- Tissue disaggregation and isolation of specific cell types from transgenic Xenopus appendages for transcriptional analysis by FACS.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Article
- The stem-like Stat3-responsive cells of zebrafish intestine are Wnt/β-catenin dependent.Development (Cambridge, England) · 2020Article
- Copper Regulates the Susceptibility of Zebrafish Larvae to Inflammatory Stimuli by Controlling Neutrophil/Macrophage Survival.Frontiers in immunology · 2019Article
- An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae.MethodsX · 2018Article
- MicroRNA miR-29 controls a compensatory response to limit neuronal iron accumulation during adult life and aging.BMC biology · 2017Article
- Opportunities in the design and application of RNA for gene expression control.Nucleic acids research · 2016Review
- A Numerical Simulation of Cell Separation by Simplified Asymmetric Pinched Flow Fractionation.Computational and mathematical methods in medicine · 2016Article
- RFX transcription factors are essential for hearing in mice.Nature communications · 2015Article
- Getting Down to Specifics: Profiling Gene Expression and Protein-DNA Interactions in a Cell Type-Specific Manner.Advances in genetics · 2015Review
- Dynamic gene expression by putative hair-cell progenitors during regeneration in the zebrafish lateral line.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
Transgenic lines carrying fluorescent reporter genes like GFP have been of great value in the elucidation of developmental features and physiological processes in various animal models, including zebrafish. The lateral line (LL), which is a fish specific superficial sensory organ, is an emerging organ model for studying complex cellular processes in the context of the whole living animal. Cell migration, mechanosensory cell development/differentiation and regeneration are some examples. This sensory system is made of superficial and sparse small sensory patches called neuromasts, with less than 50 cells in any given patch. The paucity of cells is a real problem in any effort to characterize those cells at the transcriptional level. We describe here a method which we applied to efficiently separate subpopulation of cells of the LL, using two distinct stable transgenic zebrafish lines, Tg(cldnb:gfp) and Tg(tnks1bp1:EGFP). In both cases, the GFP positive (GFP+) cells were separated from the remainder of the animal by using a Fluorescent Activated Cell Sorter (FACS). The transcripts of the GFP+ cells were subsequently analyzed on gene expression microarrays. The combination of FACS and microarrays is an efficient method to establish a transcriptional signature for discrete cell populations which would otherwise be masked in whole animal preparation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.