ArticleBiophysical journal2013
Minimal effect of lipid charge on membrane miscibility phase behavior in three ternary systems.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed.
- Apical and basolateral plasma membranes in epithelial cells have distinct lipidomes and biophysical properties.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Apical and basolateral plasma membranes in epithelial cells have distinct lipidomes and biophysical properties.bioRxiv : the preprint server for biology · 2025Article
- Several common methods of making vesicles (except an emulsion method) capture intended lipid ratios.Biophysical journal · 2024Article
- Coupling liquid phases in 3D condensates and 2D membranes: Successes, challenges, and tools.Biophysical journal · 2024Review
- Physical Concept to Explain the Regulation of Lipid Membrane Phase Separation under Isothermal Conditions.Life (Basel, Switzerland) · 2023Review
- Remodeling of yeast vacuole membrane lipidomes from the log (one phase) to stationary stage (two phases).Biophysical journal · 2023Article
- Article
- Examining the Effect of Charged Lipids on Mitochondrial Outer Membrane Dynamics Using Atomistic Simulations.Biomolecules · 2022Article
- Direct imaging of liquid domains in membranes by cryo-electron tomography.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Negatively Charged Lipids Exhibit Negligible Effects on the Water Repellency of Montmorillonite Films.ACS omega · 2020Article
- Principles and Applications of Biological Membrane Organization.Annual review of biophysics · 2020Review
- Effects of Passive Phospholipid Flip-Flop and Asymmetric External Fields on Bilayer Phase Equilibria.Biophysical journal · 2018Article
- Reorganization of Ternary Lipid Mixtures of Nonphosphorylated Phosphatidylinositol Interacting with Angiomotin.The journal of physical chemistry. B · 2018Article
- Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy.Journal of visualized experiments : JoVE · 2017Article
- Phase-Separated Liposomes Enhance the Efficiency of Macromolecular Delivery to the Cellular Cytoplasm.Cellular and molecular bioengineering · 2017Article
- n-Alcohol Length Governs Shift in LBiophysical journal · 2017Article
- Tuning the photoexcitation response of cyanobacterial Photosystem I via reconstitution into Proteoliposomes.Scientific reports · 2017Article
- cDICE method produces giant lipid vesicles under physiological conditions of charged lipids and ionic solutions.Soft matter · 2016Article
- Solution Asymmetry and Salt Expand Fluid-Fluid Coexistence Regions of Charged Membranes.Biophysical journal · 2016Article
- Steric Pressure among Membrane-Bound Polymers Opposes Lipid Phase Separation.Langmuir : the ACS journal of surfaces and colloids · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Giant unilamellar vesicles composed of a ternary mixture of phospholipids and cholesterol exhibit coexisting liquid phases over a range of temperatures and compositions. A significant fraction of lipids in biological membranes are charged. Here, we present phase diagrams of vesicles composed of phosphatidylcholine (PC) lipids, which are zwitterionic; phosphatidylglycerol (PG) lipids, which are anionic; and cholesterol (Chol). Specifically, we use DiPhyPG-DPPC-Chol and DiPhyPC-DPPG-Chol. We show that miscibility in membranes containing charged PG lipids occurs over similarly high temperatures and broad lipid compositions as in corresponding membranes containing only uncharged lipids, and that the presence of salt has a minimal effect. We verified our results in two ways. First, we used mass spectrometry to ensure that charged PC/PG/Chol vesicles formed by gentle hydration have the same composition as the lipid stocks from which they are made. Second, we repeated the experiments by substituting phosphatidylserine for PG as the charged lipid and observed similar phenomena. Our results consistently support the view that monovalent charged lipids have only a minimal effect on lipid miscibility phase behavior in our system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.