ArticleMolecular ecology resources2013
Protocols for dry DNA storage and shipment at room temperature.
Article in Molecular ecology resources, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 72 citations in OpenAlex.
- Chitosan oligosaccharide lactate (COL) chosen from different derivatives protects DNA against environmental stressors.Analytical and bioanalytical chemistry · 2026Article
- Evaluation of ensilication technology for ambient DNA preservation.NAR molecular medicine · 2026Article
- Polymer Coating for the Long-Term Storage of Immobilized DNA.ACS sensors · 2025Article
- Optimal storage time and temperature of human oral samples to minimize microbiome changes: A scoping review.The Japanese dental science review · 2024Article
- Desiccated Cyanobacteria Serve As Efficient Plasmid DNA Carriers in Space Flight.ACS synthetic biology · 2024Article
- Effects of storage conditions on the performance of an electrochemical aptamer-based sensor.Sensors & diagnostics · 2024Article
- Review
- Parallel molecular computation on digital data stored in DNA.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Continuous molecular monitoring of human dermal interstitial fluid with microneedle-enabled electrochemical aptamer sensors.Lab on a chip · 2023Article
- DNA Data Storage.Biotech (Basel (Switzerland)) · 2023Review
- Design considerations for advancing data storage with synthetic DNA for long-term archiving.Materials today. Bio · 2022Review
- Long-term stabilization of DNA at room temperature using a one-step microwave assisted process.Emergent materials · 2022Article
- Enhanced delivery efficiency and sustained release of biopharmaceuticals by complexation-based gel encapsulated coated microneedles: rhIFNα-1b example.Asian journal of pharmaceutical sciences · 2021Article
- Uncertainties in synthetic DNA-based data storage.Nucleic acids research · 2021Review
- DNA stability: a central design consideration for DNA data storage systems.Nature communications · 2021Review
- DNA degradation in fish: Practical solutions and guidelines to improve DNA preservation for genomic research.Ecology and evolution · 2020Article
- DNA storage: research landscape and future prospects.National science review · 2020Article
- A reference library for Canadian invertebrates with 1.5 million barcodes, voucher specimens, and DNA samples.Scientific data · 2019Article
- Molecular digital data storage using DNA.Nature reviews. Genetics · 2019Review
- Ensuring the Safety and Security of Frozen Lung Cancer Tissue Collections through the Encapsulation of Dried DNA.Cancers · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The globalization of DNA barcoding will require core analytical facilities to develop cost-effective, efficient protocols for the shipment and archival storage of DNA extracts and PCR products. We evaluated three dry-state DNA stabilization systems: commercial Biomatrica(®) DNAstable(®) plates, home-made trehalose and polyvinyl alcohol (PVA) plates on 96-well panels of insect DNA stored at 56 °C and at room temperature. Controls included unprotected samples that were stored dry at room temperature and at 56 °C, and diluted samples held at 4 °C and at -20 °C. PCR and selective sequencing were performed over a 4-year interval to test the condition of DNA extracts. Biomatrica(®) provided better protection of DNA at 56 °C and at room temperature than trehalose and PVA, especially for diluted samples. PVA was the second best protectant after Biomatrica(®) at room temperature, whereas trehalose was the second best protectant at 56 °C. In spite of lower PCR success, the DNA stored at -20 °C yielded longer sequence reads and stronger signal, indicating that temperature is a crucial factor for DNA quality which has to be considered especially for long-term storage. Although it is premature to advocate a transition to DNA storage at room temperature, dry storage provides an additional layer of security for frozen samples, protecting them from degradation in the event of freezer failure. All three forms of DNA preservation enable shipment of dry DNA and PCR products between barcoding facilities.
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