ArticleBiology open2013
Quantitative single cell analysis of cell population dynamics during submandibular salivary gland development and differentiation.
Article in Biology open, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
51 citing papers in PubMed, 74 citations in OpenAlex.
- Targeted regeneration of post-radiation epithelium without promoting cancer recurrence.Research square · 2026Article
- Embryonic Mammary Gland Morphogenesis.Advances in experimental medicine and biology · 2025Review
- Submandibular gland epithelial development and the importance of junctions.Tissue barriers · 2023Review
- Autologous mesenchymal stem cells offer a new paradigm for salivary gland regeneration.International journal of oral science · 2023Review
- Neurotrophin signaling is a central mechanism of salivary dysfunction after irradiation that disrupts myoepithelial cells.NPJ Regenerative medicine · 2023Article
- Identifying Fibrogenic Cells Following Salivary Gland Obstructive Injury.bioRxiv : the preprint server for biology · 2023Article
- A cellular hierarchy of Notch and Kras signaling controls cell fate specification in the developing mouse salivary gland.Developmental cell · 2023Article
- Identifying fibrogenic cells following salivary gland obstructive injury.Frontiers in cell and developmental biology · 2023Article
- Bulk RNA-seq and scRNA-seq analysis reveal an activation of immune response and compromise of secretory function in major salivary glands of obese mice.Computational and structural biotechnology journal · 2023Article
- Salivary gland function, development, and regeneration.Physiological reviews · 2022Review
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- Comparing development and regeneration in the submandibular gland highlights distinct mechanisms.Journal of anatomy · 2021Article
- Organoid Models for Salivary Gland Biology and Regenerative Medicine.Stem cells international · 2021Review
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- Article
- VDR in salivary gland homeostasis and cancer.The Journal of steroid biochemistry and molecular biology · 2020Article
- Tracing tumorigenesis in a solid tumor model at single-cell resolution.Nature communications · 2020Article
- Regional Differences following Partial Salivary Gland Resection.Journal of dental research · 2020Article
- ROCK inhibitor increases proacinar cells in adult salivary gland organoids.Stem cell research · 2019Article
- Concise Review: A Critical Evaluation of Criteria Used to Define Salivary Gland Stem Cells.Stem cells (Dayton, Ohio) · 2019Review
Corrections and comments
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Authors and funding
16 authors at 4 institutions in 1 country.
Funding
Abstract
Epithelial organ morphogenesis involves reciprocal interactions between epithelial and mesenchymal cell types to balance progenitor cell retention and expansion with cell differentiation for evolution of tissue architecture. Underlying submandibular salivary gland branching morphogenesis is the regulated proliferation and differentiation of perhaps several progenitor cell populations, which have not been characterized throughout development, and yet are critical for understanding organ development, regeneration, and disease. Here we applied a serial multiplexed fluorescent immunohistochemistry technology to map the progressive refinement of the epithelial and mesenchymal cell populations throughout development from embryonic day 14 through postnatal day 20. Using computational single cell analysis methods, we simultaneously mapped the evolving temporal and spatial location of epithelial cells expressing subsets of differentiation and progenitor markers throughout salivary gland development. We mapped epithelial cell differentiation markers, including aquaporin 5, PSP, SABPA, and mucin 10 (acinar cells); cytokeratin 7 (ductal cells); and smooth muscle α-actin (myoepithelial cells) and epithelial progenitor cell markers, cytokeratin 5 and c-kit. We used pairwise correlation and visual mapping of the cells in multiplexed images to quantify the number of single- and double-positive cells expressing these differentiation and progenitor markers at each developmental stage. We identified smooth muscle α-actin as a putative early myoepithelial progenitor marker that is expressed in cytokeratin 5-negative cells. Additionally, our results reveal dynamic expansion and redistributions of c-kit- and K5-positive progenitor cell populations throughout development and in postnatal glands. The data suggest that there are temporally and spatially discreet progenitor populations that contribute to salivary gland development and homeostasis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.