ArticleThe Journal of biological chemistry2013
Danio rerio αE-catenin is a monomeric F-actin binding protein with distinct properties from Mus musculus αE-catenin.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 32 citations in OpenAlex.
- Metabolic reprogramming in response to cell mechanics.Biology of the cell · 2023Review
- Multi-level Force-dependent Allosteric Enhancement of αE-catenin Binding to F-actin by Vinculin.Journal of molecular biology · 2023Article
- Article
- α-catenin switches between a slip and an asymmetric catch bond with F-actin to cooperatively regulate cell junction fluidity.Nature communications · 2022Article
- Cortical tension initiates the positive feedback loop between cadherin and F-actin.Biophysical journal · 2022Article
- Binding partner- and force-promoted changes in αE-catenin conformation probed by native cysteine labeling.Scientific reports · 2019Article
- Article
- α-Catenin Structure and Nanoscale Dynamics in Solution and in Complex with F-Actin.Biophysical journal · 2018Article
- Analysis of a vinculin homolog in a sponge (phylum Porifera) reveals that vertebrate-like cell adhesions emerged early in animal evolution.The Journal of biological chemistry · 2018Article
- α-Catenin homodimers are recruited to phosphoinositide-activated membranes to promote adhesion.The Journal of cell biology · 2017Article
- Cell-cell adhesion in metazoans relies on evolutionarily conserved features of the α-catenin·β-catenin-binding interface.The Journal of biological chemistry · 2017Article
- Measuring Protein Binding to F-actin by Co-sedimentation.Journal of visualized experiments : JoVE · 2017Article
- Structural and functional characterization ofThe Journal of biological chemistry · 2017Article
- Article
- Characterization of the Cadherin-Catenin Complex of the Sea Anemone Nematostella vectensis and Implications for the Evolution of Metazoan Cell-Cell Adhesion.Molecular biology and evolution · 2016Article
- αT-Catenin Is a Constitutive Actin-binding α-Catenin That Directly Couples the Cadherin·Catenin Complex to Actin Filaments.The Journal of biological chemistry · 2016Article
- Purification, crystallization and initial crystallographic analysis of the α-catenin homologue HMP-1 from Caenorhabditis elegans.Acta crystallographica. Section F, Structural biology communications · 2016Article
- Reevaluating αE-catenin monomer and homodimer functions by characterizing E-cadherin/αE-catenin chimeras.The Journal of cell biology · 2015Article
- Cell adhesion. The minimal cadherin-catenin complex binds to actin filaments under force.Science (New York, N.Y.) · 2014Article
- Structural and thermodynamic characterization of cadherin·β-catenin·α-catenin complex formation.The Journal of biological chemistry · 2014Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
It is unknown whether homologs of the cadherin·catenin complex have conserved structures and functions across the Metazoa. Mammalian αE-catenin is an allosterically regulated actin-binding protein that binds the cadherin·β-catenin complex as a monomer and whose dimerization potentiates F-actin association. We tested whether these functional properties are conserved in another vertebrate, the zebrafish Danio rerio. Here we show, despite 90% sequence identity, that Danio rerio and Mus musculus αE-catenin have striking functional differences. We demonstrate that D. rerio αE-catenin is monomeric by size exclusion chromatography, native PAGE, and small angle x-ray scattering. D. rerio αE-catenin binds F-actin in cosedimentation assays as a monomer and as an α/β-catenin heterodimer complex. D. rerio αE-catenin also bundles F-actin, as shown by negative stained transmission electron microscopy, and does not inhibit Arp2/3 complex-mediated actin nucleation in bulk polymerization assays. Thus, core properties of α-catenin function, F-actin and β-catenin binding, are conserved between mouse and zebrafish. We speculate that unique regulatory properties have evolved to match specific developmental requirements.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.