ArticleGenes & development2013
Sex-lethal promotes nuclear retention of msl2 mRNA via interactions with the STAR protein HOW.
Article in Genes & development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Held out wings RNA binding activity in the cytoplasm during early spermatogenesis.Communications biology · 2026Article
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- Integration of a multi-omics stem cell differentiation dataset using a dynamical model.PLoS genetics · 2023Article
- Genomic and functional conservation of lncRNAs: lessons from flies.Mammalian genome : official journal of the International Mammalian Genome Society · 2022Review
- Channel nuclear pore protein 54 directs sexual differentiation and neuronal wiring of female reproductive behaviors in Drosophila.BMC biology · 2021Article
- Pseudo-RNA-Binding Domains Mediate RNA Structure Specificity in Upstream of N-Ras.Cell reports · 2020Article
- Progressive dosage compensation during Drosophila embryogenesis is reflected by gene arrangement.EMBO reports · 2019Article
- Hrp48 and eIF3d contribute to msl-2 mRNA translational repression.Nucleic acids research · 2018Article
- Article
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- Intron retention as a component of regulated gene expression programs.Human genetics · 2017Review
- Differential intron retention inScience advances · 2017Article
- Promiscuity in post-transcriptional control of gene expression: Drosophila sex-lethal and its regulatory partnerships.FEBS letters · 2017Review
- The expanding universe of ribonucleoproteins: of novel RNA-binding proteins and unconventional interactions.Pflugers Archiv : European journal of physiology · 2016Review
- Sxl-Dependent, tra/tra2-Independent Alternative Splicing of the Drosophila melanogaster X-Linked Gene found in neurons.G3 (Bethesda, Md.) · 2015Article
- Mechanisms of x chromosome dosage compensation.Journal of genomics · 2015Review
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3 authors.
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Abstract
Female-specific repression of male-specific-lethal-2 (msl2) mRNA in Drosophila melanogaster provides a paradigm for coordinated control of gene expression by RNA-binding complexes. Repression is orchestrated by Sex-lethal (SXL), which binds to the 5' and 3' untranslated regions (UTRs) of the mRNA and inhibits splicing in the nucleus and subsequent translation in the cytoplasm. Here we show that SXL ensures msl2 silencing by yet a third mechanism that involves inhibition of nucleocytoplasmic transport of msl2 mRNA. To identify SXL cofactors in msl2 regulation, we devised a two-step purification method termed GRAB (GST pull-down and RNA affinity binding) and identified Held-Out-Wings (HOW) as a component of the msl2 5' UTR-associated complex. HOW directly interacts with SXL and binds to two sequence elements in the msl2 5' UTR. Depletion of HOW reduces the capacity of SXL to repress the expression of msl2 reporters without affecting SXL-mediated regulation of splicing or translation. Instead, HOW is required for SXL to retain msl2 transcripts in the nucleus. Cooperation with SXL confers a sex-specific role to HOW. Our results uncover a novel function of SXL in nuclear mRNA retention and identify HOW as a mediator of this function.
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