ArticleJournal of cell science2013
The DNA damage checkpoint protein RAD9A is essential for male meiosis in the mouse.
Article in Journal of cell science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 30 citations in OpenAlex.
- Co-Phosphoregulatory Network Underlying Functional Coherence of TLK1 and TLK2 Kinase Paralogs.International journal of molecular sciences · 2026Article
- Parasitic castration by a viral protein tyrosine phosphatase targeting the host cell cycle checkpoint protein Rad9A.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Separable roles of the DNA damage response kinase Mec1ATR and its activator Rad24RAD17 during meiotic recombination.PLoS genetics · 2024Article
- Multi-omic human pancreatic islet endoplasmic reticulum and cytokine stress response mapping provides type 2 diabetes genetic insights.Cell metabolism · 2024Article
- Transcriptional profiles analysis of effects ofFrontiers in cellular and infection microbiology · 2024Article
- Double strand DNA breaks in sperm: the bad guy in the crowd.Journal of assisted reproduction and genetics · 2023Article
- Multiple 9-1-1 complexes promote homolog synapsis, DSB repair, and ATR signaling during mammalian meiosis.eLife · 2022Article
- Meiotic sex chromosome inactivation and the XY body: a phase separation hypothesis.Cellular and molecular life sciences : CMLS · 2021Review
- Identification of key genes affecting porcine fat deposition based on co-expression network analysis of weighted genes.Journal of animal science and biotechnology · 2021Article
- ATR signaling in mammalian meiosis: From upstream scaffolds to downstream signaling.Environmental and molecular mutagenesis · 2020Review
- BRUCE preserves genomic stability in the male germline of mice.Cell death and differentiation · 2020Article
- Genome Wide Analysis for Growth at Two Growth Stages in A New Fast-Growing Common Carp Strain (Cyprinus carpio L.).Scientific reports · 2020Article
- Rad9a is involved in chromatin decondensation and post-zygotic embryo development in mice.Cell death and differentiation · 2019Article
- Pold3 is required for genomic stability and telomere integrity in embryonic stem cells and meiosis.Nucleic acids research · 2018Article
- Overexpression of Hdac6 extends reproductive lifespan in mice.Protein & cell · 2017Article
- MiR-2425-5p targets RAD9A and MYOG to regulate the proliferation and differentiation of bovine skeletal muscle-derived satellite cells.Scientific reports · 2017Article
- Rad9a is required for spermatogonia differentiation in mice.Oncotarget · 2016Article
- Article
- Genome Protection by the 9-1-1 Complex Subunit HUS1 Requires Clamp Formation, DNA Contacts, and ATR Signaling-independent Effector Functions.The Journal of biological chemistry · 2015Article
- The meiotic checkpoint network: step-by-step through meiotic prophase.Cold Spring Harbor perspectives in biology · 2014Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
In mitotic cells, RAD9A functions in repairing DNA double-strand breaks (DSBs) by homologous recombination and facilitates the process by cell cycle checkpoint control in response to DNA damage. DSBs occur naturally in the germline during meiosis but whether RAD9A participates in repairing such breaks is not known. In this study, we determined that RAD9A is indeed expressed in the male germ line with a peak of expression in late pachytene and diplotene stages, and the protein was found associated with the XY body. As complete loss of RAD9A is embryonic lethal, we constructed and characterized a mouse strain with Stra8-Cre driven germ cell-specific ablation of Rad9a beginning in undifferentiated spermatogonia in order to assess its role in spermatogenesis. Adult mutant male mice were infertile or sub-fertile due to massive loss of spermatogenic cells. The onset of this loss occurs during meiotic prophase, and there was an increase in the numbers of apoptotic spermatocytes as determined by TUNEL. Spermatocytes lacking RAD9A usually arrested in meiotic prophase, specifically in pachytene. The incidence of unrepaired DNA breaks increased, as detected by accumulation of γH2AX and DMC1 foci on the axes of autosomal chromosomes in pachytene spermatocytes. The DNA topoisomerase IIβ-binding protein 1 (TOPBP1) was still localized to the sex body, albeit with lower intensity, suggesting that RAD9A may be dispensable for sex body formation. We therefore show for the first time that RAD9A is essential for male fertility and for repair of DNA DSBs during meiotic prophase I.
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