ArticleCurrent protocols in chemical biology2013
Profiling protein methylation with cofactor analog containing terminal alkyne functionality.
Article in Current protocols in chemical biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Biocompatible Chemistry: A Plug-and-Play Toolbox for Chemical Biology Research.Chembiochem : a European journal of chemical biology · 2025Review
- Decoding the protein methylome: Identification, validation, and functional insights.Bioorganic & medicinal chemistry · 2025Review
- Chemical proteomics approaches for protein post-translational modification studies.Biochimica et biophysica acta. Proteins and proteomics · 2024Review
- Functionalised Cofactor Mimics for Interactome Discovery and Beyond.Angewandte Chemie (International ed. in English) · 2022Review
- Profiling and Validation of Live-Cell Protein Methylation with Engineered Enzymes and Methionine Analogues.Current protocols · 2021Article
- Methylation of dual-specificity phosphatase 4 controls cell differentiation.Cell reports · 2021Article
- Generating orthogonal glycosyltransferase and nucleotide sugar pairs as next-generation glycobiology tools.Current opinion in chemical biology · 2021Review
- Fine-tuning of lysine side chain modulates the activity of histone lysine methyltransferases.Scientific reports · 2020Article
- Chemical and Biochemical Perspectives of Protein Lysine Methylation.Chemical reviews · 2018Review
- Mapping the Binding Site of BMS-708163 on γ-Secretase with Cleavable Photoprobes.Cell chemical biology · 2017Article
- Kinetic isotope effects reveal early transition state of protein lysine methyltransferase SET8.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Chemical Proteomic Profiling of Human Methyltransferases.Journal of the American Chemical Society · 2016Article
- Sensing Enzymatic Activity by Exposure and Selection of DNA-Encoded Probes.Angewandte Chemie (International ed. in English) · 2016Article
- SAM/SAH Analogs as Versatile Tools for SAM-Dependent Methyltransferases.ACS chemical biology · 2016Review
- A sensitive mass spectrum assay to characterize engineered methionine adenosyltransferases with S-alkyl methionine analogues as substrates.Analytical biochemistry · 2014Article
- Profiling substrates of protein arginine N-methyltransferase 3 with S-adenosyl-L-methionine analogues.ACS chemical biology · 2014Article
- A journey toward Bioorthogonal Profiling of Protein Methylation inside living cells.Current opinion in chemical biology · 2013Review
- Expanding the structural diversity of polyketides by exploring the cofactor tolerance of an inline methyltransferase domain.Organic letters · 2013Article
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Authors and funding
4 authors.
Funding
Abstract
Enzymatic transmethylation from the cofactor S-adenosyl-L-methionine (SAM) to biological molecules has recently garnered increased attention because of the diversity of possible substrates and implications in normal biology and diseases. To reveal the substrates of protein methyltransferases (PMTs), the present article focuses on an alkyne-containing SAM mimic, Se-adenosyl-L-selenomethionine (ProSeAM), and a cleavable azido-azo-biotin probe to profile the targets of endogenous PMTs in cellular contexts. This article describes the stepwise preparation of cell lysates containing active, endogenous PMTs and subsequent target labeling with ProSeAM. The article continues with the enrichment of the ProSeAM-labeled proteins with the azido-azo biotin probe as a pulldown reagent and the subsequent reductive elution with sodium dithionate for proteomic analysis. The protocols provided here were formulated for ProSeAM as a profiling reagent but can be applied to other terminal-alkyne-containing SAM analog cofactors.
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