ArticleOptics express2013
Wide field-of-view Talbot grid-based microscopy for multicolor fluorescence imaging.
Article in Optics express, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Compact on-chip fluorescence microscope for dynamic imaging of cellular processes and biomimetic systems.JPhys photonics · 2026Article
- Speckle-structured illumination for 3D phase and fluorescence computational microscopy.Biomedical optics express · 2019Article
- Computational structured illumination for high-content fluorescence and phase microscopy.Biomedical optics express · 2019Article
- Large field-of-view phase and fluorescence mesoscope with microscopic resolution.Journal of biomedical optics · 2019Article
- Highly sensitive lens-free fluorescence imaging device enabled by a complementary combination of interference and absorption filters.Biomedical optics express · 2018Article
- Article
- Multilayer fluorescence imaging on a single-pixel detector.Biomedical optics express · 2016Article
- Compact Wireless Microscope for In-Situ Time Course Study of Large Scale Cell Dynamics within an Incubator.Scientific reports · 2015Article
- Full-color structured illumination optical sectioning microscopy.Scientific reports · 2015Article
- 0.5 gigapixel microscopy using a flatbed scanner.Biomedical optics express · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The capability to perform multicolor, wide field-of-view (FOV) fluorescence microscopy imaging is important in screening and pathology applications. We developed a microscopic slide-imaging system that can achieve multicolor, wide FOV, fluorescence imaging based on the Talbot effect. In this system, a light-spot grid generated by the Talbot effect illuminates the sample. By tilting the excitation beam, the Talbot-focused spot scans across the sample. The images are reconstructed by collecting the fluorescence emissions that correspond to each focused spot with a relay optics arrangement. The prototype system achieved an FOV of 12 × 10 mm(2) at an acquisition time as fast as 23 s for one fluorescence channel. The resolution is fundamentally limited by spot size, with a demonstrated full-width at half-maximum spot diameter of 1.2 μm. The prototype was used to nimage green fluorescent beads, double-stained human breast cancer SK-BR-3 cells, Giardia lamblia cysts, and the Cryptosporidium parvum oocysts. This imaging method is scalable and simple for implementation of high-speed wide FOV fluorescence microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.