ArticlePlant methods2013
A fluorometric assay for trehalose in the picomole range.
Article in Plant methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 81 citations in OpenAlex.
- Trehalose priming enhances chromium resilience in barley plants by modulating antioxidant defense, hormonal balance, endogenous trehalose, and nutrient homeostasis.BMC plant biology · 2026Article
- The sucrose-trehalose 6-phosphate nexus is conserved in flowering plants with different phloem loading and carbon storage strategies.Journal of experimental botany · 2026Article
- Starch dynamics as an important factor in early stages of somatic embryogenesis - a review.Frontiers in plant science · 2026Review
- Nutrient stress triggers sugar-mediated carotenoid production in algal-bacterial interactions.World journal of microbiology & biotechnology · 2025Article
- Carbon and nitrogen signaling regulate FLOWERING LOCUS C and impact flowering time in Arabidopsis.Plant physiology · 2024Article
- Direct and indirect responses of the Arabidopsis transcriptome to an induced increase in trehalose 6-phosphate.Plant physiology · 2024Article
- Distributable, metabolic PET reporting of tuberculosis.Nature communications · 2024Article
- Genome-Wide Analysis of Trehalose-6-Phosphate Phosphatase Gene Family and Their Expression Profiles in Response to Abiotic Stress in Groundnut.Plants (Basel, Switzerland) · 2024Article
- Genome- and transcriptome-wide identification of trehalose-6-phosphate phosphatases (TPP) gene family and their expression patterns under abiotic stress and exogenous trehalose in soybean.BMC plant biology · 2023Article
- Genome-Wide Identification and Analysis of Stress Response of Trehalose-6-Phosphate Synthase and Trehalose-6-Phosphate Phosphatase Genes in Quinoa.International journal of molecular sciences · 2023Article
- Triose phosphate export from chloroplasts and cellular sugar content regulate anthocyanin biosynthesis during high light acclimation.Plant communications · 2023Article
- Mathematical Modeling Reveals That Sucrose Regulates Leaf Senescence via Dynamic Sugar Signaling Pathways.International journal of molecular sciences · 2022Article
- Genome-wide analysis of trehalose-6-phosphate phosphatases (TPP) gene family in wheat indicates their roles in plant development and stress response.BMC plant biology · 2022Article
- Parallel evolution of trehalose production machinery in anhydrobiotic animals via recurrent gene loss and horizontal transfer.Open biology · 2021Article
- A needle-type micro-sampling device for collecting nanoliter sap sample from plants.Analytical and bioanalytical chemistry · 2021Article
- Metabolic engineering of osmoprotectants to elucidate the mechanism(s) of salt stress tolerance in crop plants.Planta · 2021Review
- Combining Genome-Wide Gene Expression Analysis (RNA-seq) and a Gene Editing Platform (CRISPR-Cas9) to Uncover the Selectively Pro-oxidant Activity of Aurone Compounds AgainstFrontiers in microbiology · 2021Article
- Trehalose-6-phosphate phosphatase E modulates ABA-controlled root growth and stomatal movement in Arabidopsis.Journal of integrative plant biology · 2020Article
- Enhancing Sustainability by Improving Plant Salt Tolerance through Macro- and Micro-Algal Biostimulants.Biology · 2020Review
- Carbohydrate Metabolism and Signaling in Squash Nectaries and Nectar Throughout Floral Maturation.Plant physiology · 2019Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTrehalose is a non-reducing disaccharide that is used as an osmolyte, transport sugar, carbon reserve and stress protectant in a wide range of organisms. In plants, trehalose 6-phosphate (Tre6P), the intermediate of trehalose biosynthesis, is thought to be a signal of sucrose status. Trehalose itself may play a role in pathogenic and symbiotic plant-microbe interactions, in responses to abiotic stress and in developmental signalling, but its precise functions are unknown. A major obstacle to investigating its function is the technical difficulty of measuring the very low levels of trehalose usually found in plant tissues, as most of the established trehalose assays lack sufficient specificity and/or sensitivity.
resultsA kinetic assay for trehalose was established using recombinant Escherichia coli cytoplasmic trehalase (treF), which was shown to be highly specific for trehalose. Hydrolysis of trehalose to glucose is monitored fluorometrically and the trehalose content of the tissue extract is determined from an internal calibration curve. The assay is linear for 0.2-40 pmol trehalose, and recoveries of trehalose were ≥88%. A. thaliana Col-0 rosettes contain about 20-30 nmol g-1FW of trehalose, increasing to about 50-60 nmol g-1FW in plants grown at 8°C. Trehalose is not correlated with sucrose content, whereas a strong correlation between Tre6P and sucrose was confirmed. The trehalose contents of ear inflorescence primordia from the maize ramosa3 mutant and wild type plants were 6.6±2.6 nmol g-1FW and 19.0±12.7 nmol g-1FW, respectively. The trehalose:Tre6P ratios in the ramosa3 and wild-type primordia were 2.43±0.85 and 6.16±3.45, respectively.
conclusionThe fluorometric assay is highly specific for trehalose and sensitive enough to measure the trehalose content of very small amounts of plant tissue. Chilling induced a 2-fold accumulation of trehalose in A. thaliana rosettes, but the levels were too low to make a substantial quantitative contribution to osmoregulation. Trehalose is unlikely to function as a signal of sucrose status. The abnormal inflorescence branching phenotype of the maize ramosa3 mutant might be linked to a decrease in trehalose levels in the inflorescence primordia or a downward shift in the trehalose:Tre6P ratio.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.