Trial reportOperative dentistry
Inactivation of matrix-bound matrix metalloproteinases by cross-linking agents in acid-etched dentin.
Trial report in Operative dentistry. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 78 citations in OpenAlex.
- Article
- Do polyphenol-rich solutions derived from tropical fruit seeds protect dentin against erosive-abrasive challenges?Clinical oral investigations · 2026Article
- Effect of applying carbodiimide combined with a two-step self-etch adhesive durability.BMC oral health · 2024Article
- Effect of Salvadora persica on resin-dentin bond stability.BMC oral health · 2024Article
- Is dentin biomodification with collagen cross-linking agents effective for improving dentin adhesion? A systematic review and meta-analysis.Restorative dentistry & endodontics · 2022Article
- Dentin Biomodification with Flavonoids and Calcium Phosphate Ion Clusters to Improve Dentin Bonding Stability.Materials (Basel, Switzerland) · 2022Article
- Proteolytic activity and degradation of bovine versus human dentin matrices.Journal of applied oral science : revista FOB · 2021Article
- Inhibition of matrix metalloproteinases: a troubleshooting for dentin adhesion.Restorative dentistry & endodontics · 2020Review
- Effect of different solutions in reversing the damage caused by radiotherapy in dentin structure.Medicina oral, patologia oral y cirugia bucal · 2020Article
- Effect of sodium ascorbate on bond strength and metalloproteinases activity in bleached dentin.Clinical, cosmetic and investigational dentistry · 2019Article
- Polyphenol uses in biomaterials engineering.Biomaterials · 2018Review
- Recent Advances in Adhesive Bonding - The Role of Biomolecules, Nanocompounds, and Bonding Strategies in Enhancing Resin Bonding to Dental Substrates.Current oral health reports · 2017Article
- Effect of dentin biomodifiers on the immediate and long-term bond strengths of a simplified etch and rinse adhesive to dentin.Restorative dentistry & endodontics · 2017Article
- Cross-linked dry bonding: A new etch-and-rinse technique.Dental materials : official publication of the Academy of Dental Materials · 2016Article
- On the stiffness of demineralized dentin matrices.Dental materials : official publication of the Academy of Dental Materials · 2016Article
- Transdentinal cytotoxicity of glutaraldehyde on odontoblast-like cells.Journal of dentistry · 2015Article
- Molecular weight and galloylation affect grape seed extract constituents' ability to cross-link dentin collagen in clinically relevant time.Dental materials : official publication of the Academy of Dental Materials · 2015Article
- Water distribution in dentin matrices: bound vs. unbound water.Dental materials : official publication of the Academy of Dental Materials · 2015Article
- Effect of EDTA Conditioning and Carbodiimide Pretreatment on the Bonding Performance of All-in-One Self-Etch Adhesives.International journal of dentistry · 2015Article
- Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
Abstract
objectivesPublished transmission electron microscopy analysis of in vitro resin-dentin bonds shows that, after 44 months, almost 70% of collagen fibrils from the hybrid layer disappear. Matrix metalloproteinases (MMPs) play an important role in that process and are thought to be the main factor responsible for the solubilization of dentin collagen. Therefore, this study aimed to evaluate the inactivation of matrix-bound MMPs by two different cross-linking agents, carbodiimide (EDC) or proanthocyanidin (PA), or the MMP-inhibitor, chlorhexidine (CHX), on acid-etched dentin using a simplified MMP assay method. MATERIALS AND
methodsDentin beams (2×1×6 mm) were obtained from mid-coronal dentin of sound third molars and randomly divided into six groups (G) according to the dentin treatment: G1: Deionized water (control); G2: 0.1 M EDC; G3: 0.5 M EDC; G4: 0.5 M EDC + 35% hydroxyethyl methacrylate (HEMA); G5: 5% PA; and G6: 2% CHX. The beams were etched for 15 seconds with 37% phosphoric acid, rinsed, and then immersed for 60 seconds in one of the treatment solutions. The data were expressed both in absorbance values at 412 nm and in MMP-9 activity equivalents. The total MMP activity of dentin was analyzed for one hour by colorimetric assay (Sensolyte). Data were submitted to Wilcoxon nonparametric test and Mann-Whitney tests (p>0.05).
resultsAll experimental cross-linking solutions significantly reduced MMP activity from 79.8% to 95.2% when compared to the control group. No difference was observed among 0.1 M EDC (84.8%), 5% PA (87.6%), and 2% CHX (79.8%). Addition of 35% HEMA to 0.5 M EDC produced inactivation (95.2%) that was similar to that of 0.5 M EDC alone (92.7%).
conclusionDentin treatment with cross-linking agents is effective to significantly reduce MMP activity. Mixing 0.5 M EDC and 35% HEMA did not influence EDC inhibitor potential.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.