Evidence map›Paper›PMID 23786589›Full record

ArticleFEMS yeast research2013

Primers for fourteen protein-coding genes and the deep phylogeny of the true yeasts.

Vassiliki Koufopanou, Jonathan Swire, Susan Lomas, Austin Burt

Abstract read
In one paragraph

Article in FEMS yeast research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Vassiliki KoufopanouDepartment of Life Sciences, Imperial College London, Ascot, UK. v.koufopanou@imperial.ac.uk
Jonathan Swire
Susan Lomas
Austin Burt

Funding

Biotechnology and Biological Sciences Research Council
6 · The paper itself

Abstract

The Saccharomycetales or 'true yeasts' consist of more than 800 described species, including many of scientific, medical and commercial importance. Considerable progress has been made in determining the phylogenetic relationships of these species, largely based on rDNA sequences, but many nodes for early-diverging lineages cannot be resolved with rDNA alone. rDNA is also not ideal for delineating recently diverged species. From published full-genome sequence data, we have identified 14 regions of protein-coding genes that can be PCR-amplified in a large proportion of a diverse collection of 25 yeast species using degenerate primers. Phylogenetic analysis of the sequences thus obtained reveals a well-resolved phylogeny of the Saccharomycetales with many branches having high bootstrap support. Analysis of published sequences from the Saccharomyces paradoxus species complex shows that these protein-coding gene fragments are also informative about genealogical relationships amongst closely related strains. Our set of protein-coding gene fragments is therefore suitable for analysing both ancient and recent evolutionary relationships amongst yeasts.

Indexed as

PhylogenyCluster AnalysisDNA, FungalDNA PrimersEvolution, MolecularMolecular Sequence DataSaccharomycetalesSequence Analysis, DNASequence HomologyDNA, FungalDNA PrimersPCR primersphylogeneticsSaccharomycetales

Identifiers

PMID23786589
PMCPMC3906836

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.