ArticleBMC microbiology2013
Genomic and phenotypic characterization of in vitro-generated Chlamydia trachomatis recombinants.
Article in BMC microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
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- Advances in genetic manipulation ofFrontiers in immunology · 2023Review
- Genomic Analysis of MSM Rectal Chlamydia trachomatis Isolates Identifies Predicted Tissue-Tropic Lineages Generated by Intraspecies Lateral Gene Transfer-Mediated Evolution.Infection and immunity · 2022Article
- The Impact of Lateral Gene Transfer inFrontiers in cellular and infection microbiology · 2022Review
- Inter-species lateral gene transfer focused on the Chlamydia plasticity zone identifies loci associated with immediate cytotoxicity and inclusion stability.Molecular microbiology · 2021Article
- The growing repertoire of genetic tools for dissecting chlamydial pathogenesis.Pathogens and disease · 2021Article
- Comprehensive genome analysis and comparisons of the swine pathogen, Chlamydia suis reveals unique ORFs and candidate host-specificity factors.Pathogens and disease · 2021Article
- Generation of Tetracycline and Rifamycin ResistantFrontiers in microbiology · 2021Article
- Markerless Gene Deletion by Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis.Journal of visualized experiments : JoVE · 2020Article
- Study on the Occurrence of Genetic Exchange Among Parasites of theFrontiers in cellular and infection microbiology · 2020Article
- Development of Transposon Mutagenesis for Chlamydia muridarum.Journal of bacteriology · 2019Article
- Chromosomal Recombination Targets inJournal of bacteriology · 2019Article
- Chlamydia pan-genomic analysis reveals balance between host adaptation and selective pressure to genome reduction.BMC genomics · 2019Article
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- The Finnish New Variant ofMicroorganisms · 2019Article
- Chlamydial Infection From Outside to Inside.Frontiers in microbiology · 2019Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
backgroundPre-genomic and post-genomic studies demonstrate that chlamydiae actively recombine in vitro and in vivo, although the molecular and cellular biology of this process is not well understood. In this study, we determined the genome sequence of twelve Chlamydia trachomatis recombinants that were generated in vitro under antibiotic selection. These strains were used to explore the process of recombination in Chlamydia spp., including analysis of candidate recombination hotspots, and to correlate known C. trachomatis in vitro phenotypes with parental phenotypes and genotypes.
resultsEach of the 190 examined recombination events was the product of homologous recombination, and no candidate targeting motifs were identified at recombination sites. There was a single deletion event in one recombinant progeny that resulted in the removal of 17.1 kilobases between two rRNA operons. There was no evidence for preference for any specific region of the chromosome for recombination, and analyses of a total of over 200 individual recombination events do not provide any support for recombination hotspots in vitro. Two measurable phenotypes were analyzed in these studies. First, the efficiency of attachment to host cells in the absence of centrifugation was examined, and this property segregated to regions of the chromosome that carry the polymorphic membrane protein (Pmp) genes. Second, the formation of secondary inclusions within cells varied among recombinant progeny, but this did not cleanly segregate to specific regions of the chromosome.
conclusionsThese experiments examined the process of recombination in C. trachomatis and identified tools that can be used to associate phenotype with genotype in recombinant progeny. There were no data supporting the hypothesis that particular nucleotide sequences are preferentially used for recombination in vitro. Selected phenotypes can be segregated by analysis of recombination, and this technology may be useful in preliminary analysis of the relationship of genetic variation to phenotypic variation in the chlamydiae.
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