ArticlePLoS neglected tropical diseases2013
A hamster-derived West Nile virus isolate induces persistent renal infection in mice.
Article in PLoS neglected tropical diseases, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 43 citations in OpenAlex.
- Inflammatory Response Associated with West Nile Neuroinvasive Disease: A Systematic Review.Viruses · 2024Pooled it
- Pharmaceutical Strategies for West Nile Virus in Europe, an Underrecognized Cause of Severe Disease and Mortality in Older Adults: From Supportive Care to Antiviral Development.Pharmaceuticals (Basel, Switzerland) · 2026Review
- Tissue tropism, pathology, and pathogenesis of West Nile virus infection in saltwater crocodile (Crocodylus porosus).PLoS neglected tropical diseases · 2025Article
- West Nile Virus (WNV): One-Health and Eco-Health Global Risks.Veterinary sciences · 2025Review
- Interleukins, Chemokines, and Tumor Necrosis Factor Superfamily Ligands in the Pathogenesis of West Nile Virus Infection.Viruses · 2023Review
- High Glucose Induces in HK2 Kidney Cells an IFN-Dependent ZIKV Antiviral Status Fueled by Viperin.Biomedicines · 2022Article
- Pathogenicity and virulence of West Nile virus revisited eight decades after its first isolation.Virulence · 2021Article
- A 20-year historical review of West Nile virus since its initial emergence in North America: Has West Nile virus become a neglected tropical disease?PLoS neglected tropical diseases · 2021Review
- Human Tibroviruses: Commensals or Lethal Pathogens?Viruses · 2020Review
- Article
- Optimizing PCR Detection of West Nile Virus from Body Fluid Specimens to Delineate Natural History in an Infected Human Cohort.International journal of molecular sciences · 2019Article
- Phenotypic and Genotypic Characterization of West Nile Virus Isolate 2004Hou3.International journal of molecular sciences · 2019Article
- Congenital Zika Syndrome and Extra-Central Nervous System Detection of Zika Virus in a Pre-term Newborn in Mexico.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2019Article
- Acute and Delayed Deaths after West Nile Virus Infection, Texas, USA, 2002-2012.Emerging infectious diseases · 2019Article
- Targeting host metabolism by inhibition of acetyl-Coenzyme A carboxylase reduces flavivirus infection in mouse models.Emerging microbes & infections · 2019Article
- Find the right sample: A study on the versatility of saliva and urine samples for the diagnosis of emerging viruses.BMC infectious diseases · 2018Review
- Recent advances in understanding West Nile virus host immunity and viral pathogenesis.F1000Research · 2018Review
- Article
- An Overview of Current Approaches Toward the Treatment and Prevention of West Nile Virus Infection.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Of Mice and Men: Protective and Pathogenic Immune Responses to West Nile virus Infection.Current tropical medicine reports · 2015Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
backgroundWest Nile virus (WNV) can persist long term in the brain and kidney tissues of humans, non-human primates, and hamsters. In this study, mice were infected with WNV strain H8912, previously cultured from the urine of a persistently infected hamster, to determine its pathogenesis in a murine host. METHODOLOGY/PRINCIPAL
findingsWe found that WNV H8912 was highly attenuated for neuroinvasiveness in mice. Following a systemic infection, viral RNA could be detected quickly in blood and spleen and much later in kidneys. WNV H8912 induced constitutive IL-10 production, upregulation of IFN-β and IL-1β expression, and a specific IgM response on day 10 post-infection. WNV H8912 persisted preferentially in kidneys with mild renal inflammation, and less frequently in spleen for up to 2.5 months post infection. This was concurrent with detectable serum WNV-specific IgM and IgG production. There were also significantly fewer WNV- specific T cells and lower inflammatory responses in kidneys than in spleen. Previous studies have shown that systemic wild-type WNV NY99 infection induced virus persistence preferentially in spleen than in mouse kidneys. Here, we noted that splenocytes of WNV H8912-infected mice produced significantly less IL-10 than those of WNV NY99-infected mice. Finally, WNV H8912 was also attenuated in neurovirulence. Following intracranial inoculation, WNV persisted in the brain at a low frequency, concurrent with neither inflammatory responses nor neuronal damage in the brain.
conclusionsWNV H8912 is highly attenuated in both neuroinvasiveness and neurovirulence in mice. It induces a low and delayed anti-viral response in mice and preferentially persists in the kidneys.
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Registered trials
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