ArticlePloS one2013
Patterning bacterial communities on epithelial cells.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed.
- Predatory bacteria as potential biofilm control and eradication agents in the food industry.Food science and biotechnology · 2023Review
- Characterization of an engineered mucus microenvironment for in vitro modeling of host-microbe interactions.Scientific reports · 2022Article
- Diffusible Signaling Factor, a Quorum-Sensing Molecule, Interferes with and Is Toxic Towards Bdellovibrio bacteriovorus 109J.Microbial ecology · 2021Article
- What can microfluidics do for human microbiome research?Biomicrofluidics · 2020Article
- A review of co-culture models to study the oral microenvironment and disease.Journal of oral microbiology · 2020Review
- Attack-Phase Bdellovibrio bacteriovorus Responses to Extracellular Nutrients Are Analogous to Those Seen During Late Intraperiplasmic Growth.Microbial ecology · 2017Article
- Combined Application of Bacterial Predation and Violacein to Kill Polymicrobial Pathogenic Communities.Scientific reports · 2017Article
- Serum albumin and osmolality inhibit Bdellovibrio bacteriovorus predation in human serum.Scientific reports · 2017Article
- Investigating the Responses of Human Epithelial Cells to Predatory Bacteria.Scientific reports · 2016Article
- Microstencils to generate defined, multi-species patterns of bacteria.Biomicrofluidics · 2015Article
- Effects of Carbon Dioxide Aerosols on the Viability of Escherichia coli during Biofilm Dispersal.Scientific reports · 2015Article
- Bdellovibrio bacteriovorus inhibits Staphylococcus aureus biofilm formation and invasion into human epithelial cells.Scientific reports · 2014Article
- Shedding light on microbial predator-prey population dynamics using a quantitative bioluminescence assay.Microbial ecology · 2014Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Micropatterning of bacteria using aqueous two phase system (ATPS) enables the localized culture and formation of physically separated bacterial communities on human epithelial cell sheets. This method was used to compare the effects of Escherichia coli strain MG1655 and an isogenic invasive counterpart that expresses the invasin (inv) gene from Yersinia pseudotuberculosis on the underlying epithelial cell layer. Large portions of the cell layer beneath the invasive strain were killed or detached while the non-invasive E. coli had no apparent effect on the epithelial cell layer over a 24 h observation period. In addition, simultaneous testing of the localized effects of three different bacterial species; E. coli MG1655, Shigella boydii KACC 10792 and Pseudomonas sp DSM 50906 on an epithelial cell layer is also demonstrated. The paper further shows the ability to use a bacterial predator, Bdellovibriobacteriovorus HD 100, to selectively remove the E. coli, S. boydii and P. sp communities from this bacteria-patterned epithelial cell layer. Importantly, predation and removal of the P. Sp was critical for maintaining viability of the underlying epithelial cells. Although this paper focuses on a few specific cell types, the technique should be broadly applicable to understand a variety of bacteria-epithelial cell interactions.
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Registered trials
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