ArticlePloS one2013
Substrate topography determines neuronal polarization and growth in vitro.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 87 citations in OpenAlex.
- How Neuromorphic Microstructures Control In Vitro Early-Stage Neuronal Outgrowth.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A Neuroelectronic Interface with Microstructured Substrates for Spiral Ganglion Neurons Cultured In Vitro: Proof of Concept.Biosensors · 2025Article
- Review
- Nanotopographic control of actin waves and growth cone navigation in developing neurons.Frontiers in cell and developmental biology · 2025Article
- Supra- and sub-threshold intracellular-like recording of 2D and 3D neuronal networks using nanopillar electrode arrays.Microsystems & nanoengineering · 2024Article
- Recent advances in enhances peripheral nerve orientation: the synergy of micro or nano patterns with therapeutic tactics.Journal of nanobiotechnology · 2024Review
- Beyond a Transmission Cable-New Technologies to Reveal the Richness in Axonal Electrophysiology.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Review
- Substrate topography affects PC12 cell differentiation through mechanotransduction mechanisms.Mechanobiology in medicine · 2024Article
- Synergy of Nanotopography and Electrical Conductivity of PEDOT/PSS for Enhanced Neuronal Development.ACS applied materials & interfaces · 2023Article
- Regulatory Effects of Gradient Microtopographies on Synapse Formation and Neurite Growth in Hippocampal Neurons.Journal of micromechanics and microengineering : structures, devices, and systems · 2022Article
- Anisometric Microstructures to Determine Minimal Critical Physical Cues Required for Neurite Alignment.Advanced healthcare materials · 2021Article
- Understanding, engineering, and modulating the growth of neural networks: An interdisciplinary approach.Biophysics reviews · 2021Review
- Multi-curvature micropatterns unveil distinct calcium and mitochondrial dynamics in neuronal networks.Lab on a chip · 2021Article
- Neurite guidance and neuro-caging on steps and grooves in 2.5 dimensions.Nanoscale advances · 2020Article
- A Multiscale Model to Predict Neuronal Cell Deformation with Varying Extracellular Matrix Stiffness and Topography.Cellular and molecular bioengineering · 2020Article
- Cellular and Subcellular Contact Guidance on Microfabricated Substrates.Frontiers in bioengineering and biotechnology · 2020Review
- Tissue mechanics, an important regulator of development and disease.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- High-Aspect-Ratio Semiconducting Polymer Pillars for 3D Cell Cultures.ACS applied materials & interfaces · 2019Article
- Article
- Sequential Application of Discrete Topographical Patterns Enhances Derivation of Functional Mesencephalic Dopaminergic Neurons from Human Induced Pluripotent Stem Cells.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The establishment of neuronal connectivity depends on the correct initial polarization of the young neurons. In vivo, developing neurons sense a multitude of inputs and a great number of molecules are described that affect their outgrowth. In vitro, many studies have shown the possibility to influence neuronal morphology and growth by biophysical, i.e. topographic, signaling. In this work we have taken this approach one step further and investigated the impact of substrate topography in the very early differentiation stages of developing neurons, i.e. when the cell is still at the round stage and when the first neurite is forming. For this purpose we fabricated micron sized pillar structures with highly reproducible feature sizes, and analyzed neurons on the interface of flat and topographic surfaces. We found that topographic signaling was able to attract the polarization markers of mouse embryonic neurons -N-cadherin, Golgi-centrosome complex and the first bud were oriented towards topographic stimuli. Consecutively, the axon was also preferentially extending along the pillars. These events seemed to occur regardless of pillar dimensions in the range we examined. However, we found differences in neurite length that depended on pillar dimensions. This study is one of the first to describe in detail the very early response of hippocampal neurons to topographic stimuli.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.