ArticlePloS one2013
Selection of internal control genes for real-time quantitative PCR in ovary and uterus of sows across pregnancy.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- Assessment of reference genes for qRT-PCR normalization to elucidate host response to African swine fever infection.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- PPIA, HPRT1, and YWHAZ are suitable reference genes for quantitative polymerase chain reaction assay of the hypothalamic-pituitary-gonadal axis in sows.Animal bioscience · 2022Article
- Early postnatal exposure to di(2-ethylhexyl) phthalate causes sex-specific disruption of gonadal development in pigs.Reproductive toxicology (Elmsford, N.Y.) · 2021Article
- Selection of reference genes for quantitative real-time PCR normalization in European quail tissues.Molecular biology reports · 2021Article
- Serial gene co-expression network approach to mine biological meanings from integrated transcriptomes of the porcine endometrium during estrous cycle.Functional & integrative genomics · 2020Article
- Influence of heat stress on reference genes stability in heart and liver of two chickens genotypes.PloS one · 2020Article
- Selection of reference genes for measuring the expression of aiiO in Ochrobactrum quorumnocens A44 using RT-qPCR.Scientific reports · 2019Article
- Inter-genus gene expression analysis in livestock fibroblasts using reference gene validation based upon a multi-species primer set.PloS one · 2019Article
- Integrated transcriptomes throughout swine oestrous cycle reveal dynamic changes in reproductive tissues interacting networks.Scientific reports · 2018Article
- A new set of reference housekeeping genes for the normalization RT-qPCR data from the intestine of piglets during weaning.PloS one · 2018Article
- Influence of heat stress, sex and genetic groups on reference genes stability in muscle tissue of chicken.PloS one · 2017Article
- The 60S ribosomal protein L13 is the most preferable reference gene to investigate gene expression in selected organs from turkeys and chickens, in context of different infection models.Veterinary research · 2016Article
- Identifying suitable reference genes for gene expression analysis in developing skeletal muscle in pigs.PeerJ · 2016Article
- Endometrial gene expression profile of pregnant sows with extreme phenotypes for reproductive efficiency.Scientific reports · 2015Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundReproductive traits play a key role in pig production in order to reduce costs and increase economic returns. Among others, gene expression analyses represent a useful approach to study genetic mechanisms underlying reproductive traits in pigs. The application of reverse-transcription quantitative PCR requires the selection of appropriate reference genes, whose expression levels should not be affected by the experimental conditions, especially when comparing gene expression across different physiological stages.
resultsThe gene expression stability of ten potential reference genes was studied by three different methods (geNorm, NormFinder and BestKeeper) in ovary and uterus collected at five different physiological time points (heat, and 15, 30, 45 and 60 days of pregnancy). Although final ranking differed, the three algorithms gave very similar results. Thus, the most stable genes across time were TBP and UBC in uterus and TBP and HPRT1 in ovary, while HMBS and ACTB showed the less stable expression in uterus and ovary, respectively. When studied as a systematic effect, the reproductive stage did not significantly affect the expression of the candidate reference genes except at 30d and 60d of pregnancy, when a general drop in expression was observed in ovary.
conclusionsBased in our results, we propose the use of TBP, UBC and SDHA in uterus and TBP, GNB2L1 and HPRT1 in ovary for normalization of longitudinal expression studies using quantitative PCR in sows.
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