ArticlePloS one2013
Mice with hypomorphic expression of the sodium-phosphate cotransporter PiT1/Slc20a1 have an unexpected normal bone mineralization.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- Phosphate homeostasis and endocrine regulators.Pediatric nephrology (Berlin, Germany) · 2026Review
- Phosphate overload via the type III Na-dependent Pi transporter represses aortic wall elastic fiber formation.Fujita medical journal · 2024Article
- Pharmacology of Mammalian NaHandbook of experimental pharmacology · 2024Article
- Modulating Temporospatial Phosphate Equilibrium by Nanoparticulate Mineralized Collagen Materials Induces Osteogenesis via PiT-1 and PiT-2.Advanced healthcare materials · 2023Article
- Prevention of vascular calcification by the endogenous chromogranin A-derived mediator that inhibits osteogenic transdifferentiation.Basic research in cardiology · 2021Article
- Membrane Transport Proteins in Osteoclasts: The Ins and Outs.Frontiers in cell and developmental biology · 2021Review
- Histone deacetylase 4 deletion results in abnormal chondrocyte hypertrophy and premature ossification from collagen type 2α1‑expressing cells.Molecular medicine reports · 2020Article
- Review
- Slc20a1/Pit1 and Slc20a2/Pit2 are essential for normal skeletal myofiber function and survival.Scientific reports · 2020Article
- Slc20a2, Encoding the Phosphate Transporter PiT2, Is an Important Genetic Determinant of Bone Quality and Strength.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2019Article
- Expression and function of Slc34 sodium-phosphate co-transporters in skeleton and teeth.Pflugers Archiv : European journal of physiology · 2019Review
- Roles of Phosphate in Skeleton.Frontiers in endocrinology · 2019Review
- Transgenic mouse model for conditional expression of influenza hemagglutinin-tagged human SLC20A1/PIT1.PloS one · 2019Article
- Role of phosphate sensing in bone and mineral metabolism.Nature reviews. Endocrinology · 2018Review
- PiT-2, a type III sodium-dependent phosphate transporter, protects against vascular calcification in mice with chronic kidney disease fed a high-phosphate diet.Kidney international · 2018Article
- FoxO6 regulates Hippo signaling and growth of the craniofacial complex.PLoS genetics · 2018Article
- Expression of Phosphate Transporters during Dental Mineralization.Journal of dental research · 2018Article
- Loss of PiT-2 results in abnormal bone development and decreased bone mineral density and length in mice.Biochemical and biophysical research communications · 2018Article
- Skeletal Mineralization Deficits and Impaired Biogenesis and Function of Chondrocyte-Derived Matrix Vesicles in Phospho1(-/-) and Phospho1/Pi t1 Double-Knockout Mice.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2016Article
- Vitamin-D receptor agonist calcitriol reduces calcification in vitro through selective upregulation of SLC20A2 but not SLC20A1 or XPR1.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The formation of hydroxyapatite crystals and their insertion into collagen fibrils of the matrix are essential steps for bone mineralization. As phosphate is a main structural component of apatite crystals, its uptake by skeletal cells is critical and must be controlled by specialized membrane proteins. In mammals, in vitro studies have suggested that the high-affinity sodium-phosphate cotransporter PiT1 could play this role. In vivo, PiT1 expression was detected in hypertrophic chondrocytes of murine metatarsals, but its implication in bone physiology is not yet deciphered. As the complete deletion of PiT1 results in embryonic lethality at E12.5, we took advantage of a mouse model bearing two copies of PiT1 hypomorphic alleles to study the effect of a low expression of PiT1 on bone mineralization in vivo. In this report, we show that a 85% down-regulation of PiT1 in long bones resulted in a slight (6%) but significant reduction of femur length in young mice (15- and 30-day-old). However, despite a defect in alcian blue / alizarin red S and Von Kossa staining of hypomorphic 1-day-old mice, using X-rays micro-computed tomography, energy dispersive X-ray spectroscopy and histological staining techniques we could not detect differences between hypomorphic and wild-type mice of 15- to 300-days old. Interestingly, the expression of PiT2, the paralog of PiT1, was increased 2-fold in bone of PiT1 hypomorphic mice accounting for a normal phosphate uptake in mutant cells. Whether this may contribute to the absence of bone mineralization defects remains to be further deciphered.
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