ArticlePloS one2013
CD9-positive microvesicles mediate the transfer of molecules to Bovine Spermatozoa during epididymal maturation.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
62 citing papers in PubMed, 154 citations in OpenAlex.
- Seminal Extracellular Vesicles as Molecular Modulators of Male Reproductive Function.Reproduction in domestic animals = Zuchthygiene · 2026Review
- Epididymosome-Supplemented Extender Induces Changes in Morpho-Functional Traits and microRNA Levels of Post-Thaw Sperm and Improves Embryo Developmental Potential.Molecular reproduction and development · 2026Article
- Molecular Profiling of Extracellular Vesicles Isolated from Boar Reproductive Fluids.Journal of extracellular vesicles · 2026Article
- Seminal Plasma and Extracellular Vesicles as Molecular Gatekeepers: Oxidative Stress, Endocrine Crosstalk, and Biomarker Discovery in Male Infertility.Current issues in molecular biology · 2026Review
- Epididymosomes: Composition and Functions for Sperm Maturation.Advances in anatomy, embryology, and cell biology · 2026Review
- Role of Seminal Exosomes in Reproduction.Journal of cellular physiology · 2025Review
- Spatial Distribution of Extracellular Vesicles, Autofluorescence and CD9 Positivity Around Chondrocytes in the Superficial Layer of Articular Cartilage.Journal of extracellular vesicles · 2025Article
- Disruption of TEX38 impairs sperm morphogenesis and the migration of sperm into the oviduct.Communications biology · 2025Article
- Male reproductive tract extracellular vesicles display region-specific heterogeneity in mice.Reproduction (Cambridge, England) · 2025Article
- Seminal Plasma Extracellular Vesicles: Key Mediators of Intercellular Communication in Mammalian Reproductive Systems.Veterinary sciences · 2025Review
- Engineered extracellular vesicles: a breakthrough approach to overcoming sperm cryopreservation challenges.Reproductive biology and endocrinology : RB&E · 2025Review
- Conditioning period impacts the morphology and proliferative effect of extracellular vesicles derived from rat adipose tissue derived stromal cell.Journal of nanobiotechnology · 2025Article
- Circulating microRNAs in Body Fluid: "Fingerprint" RNA Snippets Deeply Impact Reproductive Biology.Reproductive sciences (Thousand Oaks, Calif.) · 2025Review
- Prominosomes - a particular class of extracellular vesicles containing prominin-1/CD133?Journal of nanobiotechnology · 2025Review
- Extracellular vesicles in seminal plasma of Sahiwal cattle bulls carry a differential abundance of sperm fertility-associated proteins for augmenting the functional quality of low-fertile bull spermatozoa.Scientific reports · 2025Article
- The extracellular vesicle tetraspanin CD63 journey from the testis through the epididymis to mature bull sperm.Scientific reports · 2024Article
- Immunophenotype profile by flow cytometry reveals different subtypes of extracellular vesicles in porcine seminal plasma.Cell communication and signaling : CCS · 2024Article
- Screening Indels from the whole genome to identify the candidates and their association with economic traits in several goat breeds.Functional & integrative genomics · 2023Article
- Extracellular vesicles from seminal plasma to improve fertilizing capacity of bulls.Reproduction & fertility · 2022Article
- Seminal extracellular vesicles subsets modulate gene expression in cumulus cells of porcine in vitro matured oocytes.Scientific reports · 2022Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Acquisition of fertilization ability by spermatozoa during epididymal transit occurs in part by the transfer of molecules from membranous vesicles called epididymosomes. Epididymosomes are heterogeneous in terms of both size and molecular composition. Exosomes and other related small membranous vesicles (30-120 nm) containing tetraspanin proteins on their surface are found in many biological fluids. In this study, we demonstrate that these vesicles are present in bovine cauda epididymal fluid as a subpopulation of epididymosomes. They contain tetraspanin CD9 in addition to other proteins involved in sperm maturation such as P25b, GliPr1L1, and MIF. In order to study the mechanism of protein transfer to sperm, DilC12-labeled unfractionated epididymosomes or CD9-positive microvesicles were coincubated with epididymal spermatozoa, and their transfer was evaluated by flow cytometry. CD9-positive microvesicles from epididymal fluid specifically transferred molecules to spermatozoa, whereas those prepared from blood were unable to do so. The CD9-positive microvesicles transferred molecules to the same sperm regions (acrosome and midpiece) as epididymosomes, with the same kinetics; however, the molecules were preferentially transferred to live sperm and, in contrast to epididymosomes, Zn(2+) did not demonstrate potentiated transfer. Tetraspanin CD9 was associated with other proteins on the membrane surface of CD9-positive microvesicles according to coimmunoprecipitation experiments. CD26 cooperated with CD9 in the molecular transfer to sperm since the amount of molecules transferred was significantly reduced in the presence of specific antibodies. In conclusion, CD9-positive microvesicles are present in bovine cauda epididymal fluid and transfer molecules to live maturing sperm in a tissue-specific manner that involves CD9 and CD26.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.