ArticlePloS one2013
Combinatorial library of improved peptide aptamers, CLIPs to inhibit RAGE signal transduction in mammalian cells.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Non-Antibody-Based Binders for the Enrichment of Proteins for Analysis by Mass Spectrometry.Biomolecules · 2021Review
- Journey to a Receptor for Advanced Glycation End Products Connection in Severe Acute Respiratory Syndrome Coronavirus 2 Infection: With Stops Along the Way in the Lung, Heart, Blood Vessels, and Adipose Tissue.Arteriosclerosis, thrombosis, and vascular biology · 2021Review
- NoPv1: a synthetic antimicrobial peptide aptamer targeting the causal agents of grapevine downy mildew and potato late blight.Scientific reports · 2020Article
- Potent Inhibitors of Mycobacterium tuberculosis Growth Identified by Using in-Cell NMR-based Screening.ACS chemical biology · 2018Article
- Real-Time In-Cell Nuclear Magnetic Resonance: Ribosome-Targeted Antibiotics Modulate Quinary Protein Interactions.Biochemistry · 2018Article
- Glycation & the RAGE axis: targeting signal transduction through DIAPH1.Expert review of proteomics · 2017Review
- Cellular mechanisms and consequences of glycation in atherosclerosis and obesity.Biochimica et biophysica acta · 2016Review
- Change in the Molecular Dimension of a RAGE-Ligand Complex Triggers RAGE Signaling.Structure (London, England : 1993) · 2016Article
- The multiple faces of RAGE--opportunities for therapeutic intervention in aging and chronic disease.Expert opinion on therapeutic targets · 2016Review
- Emerging Targets for Therapeutic Development in Diabetes and Its Complications: The RAGE Signaling Pathway.Clinical pharmacology and therapeutics · 2015Review
- Probing protein quinary interactions by in-cell nuclear magnetic resonance spectroscopy.Biochemistry · 2015Article
- Caught in action: selecting peptide aptamers against intrinsically disordered proteins in live cells.Scientific reports · 2015Article
- Synthetic biology for pharmaceutical drug discovery.Drug design, development and therapy · 2015Review
- Peptide aptamers: development and applications.Current topics in medicinal chemistry · 2015Review
- Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Peptide aptamers are small proteins containing a randomized peptide sequence embedded into a stable protein scaffold, such as Thioredoxin. We developed a robust method for building a Combinatorial Library of Improved Peptide aptamers (CLIPs) of high complexity, containing ≥3×10(10) independent clones, to be used as a molecular tool in the study of biological pathways. The Thioredoxin scaffold was modified to increase solubility and eliminate aggregation of the peptide aptamers. The CLIPs was used in a yeast two-hybrid screen to identify peptide aptamers that bind to various domains of the Receptor for Advanced Glycation End products (RAGE). NMR spectroscopy was used to identify interaction surfaces between the peptide aptamers and RAGE domains. Cellular functional assays revealed that in addition to directly interfering with known binding sites, peptide aptamer binding distal to ligand sites also inhibits RAGE ligand-induced signal transduction. This finding underscores the potential of using CLIPs to select allosteric inhibitors of biological targets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.