ArticleFrontiers in microbiology2013
Compartment-specific pH monitoring in Bacillus subtilis using fluorescent sensor proteins: a tool to analyze the antibacterial effect of weak organic acids.
Article in Frontiers in microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 52 citations in OpenAlex.
- A metabolite morphogen coordinates multicellular development inScience advances · 2026Article
- A Ratiometric pH Sensor for Gram-Positive and Gram-Negative Bacteria.Journal of the American Chemical Society · 2026Article
- Sorption of Heavy Metals (Pb, Cd, Co, and Zn) by Bacteria of the GenusInternational journal of microbiology · 2025Article
- Bacterial spore surface nanoenvironment requires a AAA+ ATPase to promote MurG function.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Methods for studying microbial acid stress responses: from molecules to populations.FEMS microbiology reviews · 2024Review
- Bacterial cell surface nanoenvironment requires a specialized chaperone to activate a peptidoglycan biosynthetic enzyme.bioRxiv : the preprint server for biology · 2023Article
- Role ofMicroorganisms · 2023Article
- Genomic versus Plasmid-Borne Expression of Germinant Receptor Proteins in Bacillus cereus Strain 14579Microorganisms · 2022Article
- Bioluminescence Color-Tuning Firefly Luciferases: Engineering and Prospects for Real-Time Intracellular pH Imaging and Heavy Metal Biosensing.Biosensors · 2022Review
- Organization and dynamics of the SpoVAEa protein and its surrounding inner membrane lipids, upon germination of Bacillus subtilis spores.Scientific reports · 2022Article
- Structural and Biochemical Analysis of the Furan Aldehyde Reductase YugJ fromInternational journal of molecular sciences · 2022Article
- DNA-Origami-Based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells.Nano letters · 2020Article
- Article
- Combinatorial pathway enzyme engineering and host engineering overcomes pyruvate overflow and enhances overproduction of N-acetylglucosamine in Bacillus subtilis.Microbial cell factories · 2019Article
- Intracellular pHluorin as Sensor for Easy Assessment of Bacteriocin-Induced Membrane-Damage inFrontiers in microbiology · 2018Article
- Article
- Intracellular pH Response to Weak Acid Stress in Individual Vegetative Bacillus subtilis Cells.Applied and environmental microbiology · 2016Article
- A pH-driven transition of the cytoplasm from a fluid- to a solid-like state promotes entry into dormancy.eLife · 2016Article
- RodZ and PgsA Play Intertwined Roles in Membrane Homeostasis ofFrontiers in microbiology · 2016Article
- Distinct effects of sorbic acid and acetic acid on the electrophysiology and metabolism of Bacillus subtilis.Applied and environmental microbiology · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The internal pH (pHi) of a living cell is one of its most important physiological parameters. To monitor the pH inside Bacillus subtilis during various stages of its life cycle, we constructed an improved version (IpHluorin) of the ratiometric, pH-sensitive fluorescent protein pHluorin by extending it at the 5' end with the first 24 bp of comGA. The new version, which showed an approximate 40% increase in fluorescence intensity, was expressed from developmental phase-specific, native promoters of B. subtilis that are specifically active during vegetative growth on glucose (PptsG) or during sporulation (PspoIIA, PspoIIID, and PsspE). Our results show strong, compartment-specific expression of IpHluorin that allowed accurate pHi measurements of live cultures during exponential growth, early and late sporulation, spore germination, and during subsequent spore outgrowth. Dormant spores were characterized by an pHi of 6.0 ± 0.3. Upon full germination the pHi rose dependent on the medium to 7.0-7.4. The presence of sorbic acid in the germination medium inhibited a rise in the intracellular pH of germinating spores and inhibited germination. Such effects were absent when acetic was added at identical concentrations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.