ArticlePLoS pathogens2013
An apicoplast localized ubiquitylation system is required for the import of nuclear-encoded plastid proteins.
Article in PLoS pathogens, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 89 citations in OpenAlex.
- The long and winding road: On the endoplasmic reticulum of Plasmodium and implications for pathogenesis.PLoS pathogens · 2026Review
- Derlin-like proteins are essential for the function of Euglena gracilis chloroplasts.Plant physiology · 2026Article
- Molecular Phylogeny of the SELMA Translocation Machinery Recounts the Evolution of Complex Photosynthetic Eukaryotes.Molecular biology and evolution · 2025Article
- Ubiquitin-activating enzyme1 (TgUAE1) acts as a key regulator of Toxoplasma gondii lytic cycle and homeostasis.Communications biology · 2025Article
- A novel YGGT family protein is localized in the apicoplast and is essential for the organelle inheritance.Frontiers in cellular and infection microbiology · 2025Article
- Plastid translocon recycling in dinoflagellates demonstrates the portability of complex plastids between hosts.Current biology : CB · 2024Article
- Plasmodium falciparum contains functional SCF and CRL4 ubiquitin E3 ligases, and CRL4 is critical for cell division and membrane integrity.PLoS pathogens · 2024Article
- An apicoplast-localized deubiquitinase contributes to the cell growth and apicoplast homeostasis of Toxoplasma gondii.Veterinary research · 2024Article
- Insights into the Cell Division ofMicroorganisms · 2023Article
- Characterisation of the OTU domain deubiquitinase complement ofLife science alliance · 2023Article
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- Reevaluation of theGenome research · 2021Article
- Endoplasmic Reticulum Stress, a Target for Drug Design and Drug Resistance in Parasitosis.Frontiers in microbiology · 2021Review
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- Ubiquitin activation is essential for schizont maturation in Plasmodium falciparum blood-stage development.PLoS pathogens · 2020Article
- Overexpression of the HECT ubiquitin ligase PfUT prolongs the intraerythrocytic cycle and reduces invasion efficiency of Plasmodium falciparum.Scientific reports · 2019Article
- Plastid-endomembrane connections in apicomplexan parasites.PLoS pathogens · 2019Review
- Wolbachia endosymbionts subvert the endoplasmic reticulum to acquire host membranes without triggering ER stress.PLoS neglected tropical diseases · 2019Article
- Host Cell Metabolism Contributes to Delayed-Death Kinetics of Apicoplast Inhibitors in Toxoplasma gondii.Antimicrobial agents and chemotherapy · 2019Article
Corrections and comments
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Authors and funding
11 authors at 6 institutions in 3 countries.
Funding
Abstract
Apicomplexan parasites are responsible for numerous important human diseases including toxoplasmosis, cryptosporidiosis, and most importantly malaria. There is a constant need for new antimalarials, and one of most keenly pursued drug targets is an ancient algal endosymbiont, the apicoplast. The apicoplast is essential for parasite survival, and several aspects of its metabolism and maintenance have been validated as targets of anti-parasitic drug treatment. Most apicoplast proteins are nuclear encoded and have to be imported into the organelle. Recently, a protein translocon typically required for endoplasmic reticulum associated protein degradation (ERAD) has been proposed to act in apicoplast protein import. Here, we show ubiquitylation to be a conserved and essential component of this process. We identify apicoplast localized ubiquitin activating, conjugating and ligating enzymes in Toxoplasma gondii and Plasmodium falciparum and observe biochemical activity by in vitro reconstitution. Using conditional gene ablation and complementation analysis we link this activity to apicoplast protein import and parasite survival. Our studies suggest ubiquitylation to be a mechanistic requirement of apicoplast protein import independent to the proteasomal degradation pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.