ArticleDevelopment (Cambridge, England)2013
A simple PCR-based strategy for estimating species-specific contributions in chimeras and xenografts.
Article in Development (Cambridge, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Cellular, Molecular, and Genetic Mechanisms of Avian Beak Development and Evolution.Annual review of genetics · 2024Review
- A novel qPCR-based technique for identifying avian sex: An illustration within embryonic craniofacial bone.Genesis (New York, N.Y. : 2000) · 2024Article
- Treatment with an inhibitor of matrix metalloproteinase 9 or cathepsin K lengthens embryonic lower jaw bone.Orthodontics & craniofacial research · 2023Article
- Application of a 3D hydrogel-based model to replace use of animals for passaging patient-derived xenografts.In vitro models · 2023Article
- Article
- Stable integration of an optimized inducible promoter system enables spatiotemporal control of gene expression throughout avian development.Biology open · 2020Article
- FGF and TGFβ signaling link form and function during jaw development and evolution.Developmental biology · 2018Article
- Neural crest and the origin of species-specific pattern.Genesis (New York, N.Y. : 2000) · 2018Review
- The taming of the neural crest: a developmental perspective on the origins of morphological covariation in domesticated mammals.Royal Society open science · 2016Review
- Neural crest-mediated bone resorption is a determinant of species-specific jaw length.Developmental biology · 2015Article
- Divergence of craniofacial developmental trajectories among avian embryos.Developmental dynamics : an official publication of the American Association of Anatomists · 2015Article
- Signals from the brain induce variation in avian facial shape.Developmental dynamics : an official publication of the American Association of Anatomists · 2015Article
- Assessing species-specific contributions to craniofacial development using quail-duck chimeras.Journal of visualized experiments : JoVE · 2014Article
- Multiple developmental mechanisms regulate species-specific jaw size.Development (Cambridge, England) · 2014Article
- Evolution of a developmental mechanism: Species-specific regulation of the cell cycle and the timing of events during craniofacial osteogenesis.Developmental biology · 2014Article
- Development of genetically flexible mouse models of sarcoma using RCAS-TVA mediated gene delivery.PloS one · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Many tissue-engineering approaches for repair and regeneration involve transplants between species. Yet a challenge is distinguishing donor versus host effects on gene expression. This study provides a simple molecular strategy to quantify species-specific contributions in chimeras and xenografts. Species-specific primers for reverse transcription quantitative real-time PCR (RT-qPCR) were designed by identifying silent mutations in quail, duck, chicken, mouse and human ribosomal protein L19 (RPL19). cDNA from different pairs of species was mixed in a dilution series and species-specific RPL19 primers were used to generate standard curves. Then quail cells were transplanted into transgenic-GFP chick and resulting chimeras were analyzed with species-specific primers. Fluorescence-activated cell sorting (FACS) confirmed that donor- and host-specific levels of RPL19 expression represent actual proportions of cells. To apply the RPL19 strategy, we measured Runx2 expression in quail-duck chimeras. Elevated Runx2 levels correlated with higher percentages of donor cells. Finally, RPL19 primers also discriminated mouse from human and chick. Thus, this strategy enables chimeras and/or xenografts to be screened rapidly at the molecular level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.