ArticleGenome biology and evolution2013
Large deletions in the pAtC58 megaplasmid of Agrobacterium tumefaciens can confer reduced carriage cost and increased expression of virulence genes.
Article in Genome biology and evolution, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
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- A dicentric bacterial chromosome requires XerC/D site-specific recombinases for resolution.Current biology : CB · 2022Article
- Experimental evolution of the megaplasmid pMPPla107 inPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Plasmid fitness costs are caused by specific genetic conflicts enabling resolution by compensatory mutation.PLoS biology · 2021Article
- A large self-transmissible resistance plasmid from Nigeria contains genes that ameliorate a carrying cost.Scientific reports · 2019Article
- Destabilization of the Tumor-Inducing Plasmid from an Octopine-TypeG3 (Bethesda, Md.) · 2019Article
- Genomic and Transcriptomic Changes that Mediate Increased Platinum Resistance in Cupriavidus metallidurans.Genes · 2019Article
- The Divided Bacterial Genome: Structure, Function, and Evolution.Microbiology and molecular biology reviews : MMBR · 2017Review
- Metabolic modelling reveals the specialization of secondary replicons for niche adaptation in Sinorhizobium meliloti.Nature communications · 2016Article
- Engineering the genome of Thermus thermophilus using a counterselectable marker.Journal of bacteriology · 2015Article
- Examination of prokaryotic multipartite genome evolution through experimental genome reduction.PLoS genetics · 2014Article
- Characterization of Rhizobium grahamii extrachromosomal replicons and their transfer among rhizobia.BMC microbiology · 2014Article
- Functions and regulation of quorum-sensing in Agrobacterium tumefaciens.Frontiers in plant science · 2014Review
- Ecological dynamics and complex interactions of Agrobacterium megaplasmids.Frontiers in plant science · 2014Review
- Mechanisms and regulation of surface interactions and biofilm formation in Agrobacterium.Frontiers in plant science · 2014Review
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4 authors.
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Abstract
The accessory plasmid pAtC58 of the common laboratory strain of Agrobacterium tumefaciens confers numerous catabolic functions and has been proposed to play a role in virulence. Genomic sequencing of evolved laboratory strains of A. tumefaciens revealed the presence of multiple deletion events in the At plasmid, with reductions in plasmid size ranging from 25% to 30% (115-194 kb). Flanking both ends of the sites of these deletions is a short-nucleotide repeat sequence that is in a single copy in the deleted plasmids, characteristic of a phage- or transposon-mediated deletion event. This repeat sequence is widespread throughout the C58 genome, but concentrated on the At plasmid, suggesting its frequency to be nonrandom. In this study, we assess the prevalence of the larger of these deletions in multiple C58 derivatives and characterize its functional significance. We find that in addition to elevating virulence gene expression, this deletion is associated with a significantly reduced carriage cost to the cell. These observations are a clear demonstration of the dynamic nature of the bacterial genome and suggest a mechanism for genetic plasticity of these costly but otherwise stable plasmids. Additionally, this phenomenon could be the basis for some of the dramatic recombination events so ubiquitous within and among megaplasmids.
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