ArticleVeterinary research2013
The synergistic necrohemorrhagic action of Clostridium perfringens perfringolysin and alpha toxin in the bovine intestine and against bovine endothelial cells.
Article in Veterinary research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 50 citations in OpenAlex.
- Toxin-neutralizing antibodies protect against Clostridium perfringens-induced necrosis in an intestinal loop model for bovine necrohemorrhagic enteritis.BMC veterinary research · 2016Trial
- Isolation, Genomic Evolution, and Pathogenicity ofAnimals : an open access journal from MDPI · 2026Article
- A lipid nanoparticle encapsulated CPA-CTD mRNA vaccine provides protection againstFrontiers in immunology · 2025Article
- The Barrier Disruption and Pyroptosis of Intestinal Epithelial Cells Caused by Perfringolysin O (PFO) fromCells · 2024Article
- Particular genomic and virulence traits associated with preterm infant-derived toxigenic Clostridium perfringens strains.Nature microbiology · 2023Article
- Clinical and Microbiological Features of Fulminant Haemolysis Caused byMicroorganisms · 2023Review
- Baicalin-aluminum alleviates necrotic enteritis in broiler chickens by inhibiting virulence factors expression ofFrontiers in cellular and infection microbiology · 2023Article
- Article
- Immunogenic and neutralization efficacy of recombinant perfringolysin O of Clostridium perfringens and its C-terminal receptor-binding domain in a murine model.Immunologic research · 2022Article
- Infection Dynamics ofFrontiers in veterinary science · 2022Article
- Intra-species diversity ofFrontiers in microbiology · 2022Article
- Establishment of a Publicly Available Core Genome Multilocus Sequence Typing Scheme for Clostridium perfringens.Microbiology spectrum · 2021Article
- Vaccination against pathogenic clostridia in animals: a review.Tropical animal health and production · 2021Review
- Occurrence and Toxicogenetic Profiling ofToxins · 2021Article
- Comparative Genomics ofFrontiers in microbiology · 2021Article
- Pathogenic Characterization ofFrontiers in microbiology · 2021Article
- Generation of recombinant baculovirus expressing atoxic C-terminal CPA toxin of Clostridium perfringens and production of specific antibodies.BMC biotechnology · 2020Article
- Amentoflavone AttenuatesFrontiers in pharmacology · 2020Article
- Genomic analysis on broiler-associatedAnimal microbiome · 2019Article
- Phylogenomic analysis of gastroenteritis-associatedMicrobial genomics · 2019Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bovine necrohemorrhagic enteritis is a major cause of mortality in veal calves. Clostridium perfringens is considered as the causative agent, but there has been controversy on the toxins responsible for the disease. Recently, it has been demonstrated that a variety of C. perfringens type A strains can induce necrohemorrhagic lesions in a calf intestinal loop assay. These results put forward alpha toxin and perfringolysin as potential causative toxins, since both are produced by all C. perfringens type A strains. The importance of perfringolysin in the pathogenesis of bovine necrohemorrhagic enteritis has not been studied before. Therefore, the objective of the current study was to evaluate the role of perfringolysin in the development of necrohemorrhagic enteritis lesions in calves and its synergism with alpha toxin. A perfringolysin-deficient mutant, an alpha toxin-deficient mutant and a perfringolysin alpha toxin double mutant were less able to induce necrosis in a calf intestinal loop assay as compared to the wild-type strain. Only complementation with both toxins could restore the activity to that of the wild-type. In addition, perfringolysin and alpha toxin had a synergistic cytotoxic effect on bovine endothelial cells. This endothelial cell damage potentially explains why capillary hemorrhages are an initial step in the development of bovine necrohemorrhagic enteritis. Taken together, our results show that perfringolysin acts synergistically with alpha toxin in the development of necrohemorrhagic enteritis in a calf intestinal loop model and we hypothesize that both toxins act by targeting the endothelial cells.
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