ArticleAnalytical chemistry2013
Toward 'omic scale metabolite profiling: a dual separation-mass spectrometry approach for coverage of lipid and central carbon metabolism.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 137 papers.
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Who cites it
137 citing papers in PubMed, 267 citations in OpenAlex.
- Acute aerobic exercise reveals that FAHFAs distinguish the metabolomes of overweight and normal-weight runners.JCI insight · 2022Trial
- Arteriovenous Blood Metabolomics: A Readout of Intra-Tissue Metabostasis.Scientific reports · 2015Trial
- Metabolism links bacterial biofilms and colon carcinogenesis.Cell metabolism · 2015Trial
- Fast and Scalable Sample Preparation for Integrated Metabolome and Exposome-Wide Association Studies (MWAS/ExWAS).Analytical chemistry · 2026Article
- Exploring the Metabolic Impact of Traumatic Brain Injury in CCI Mouse Models: A Focus on Early and Prolonged Injury Responses.International journal of molecular sciences · 2026Article
- Scanning central carbon metabolism: a HILIC-HR-TOF-MS metabolome method.Metabolomics : Official journal of the Metabolomic Society · 2026Article
- Decoding cancer across scales with metabolomics.Nature reviews. Cancer · 2026Review
- Receptor-Mitochondria Crosstalk in the Kynurenine Metabolic Pathway: Integrating Metabolomics and Clinical Mass Spectrometry.Antioxidants (Basel, Switzerland) · 2026Review
- Automated Integration and Quality Assessment of Chromatographic Peaks in LC-MS-Based Metabolomics and Lipidomics Using TARDIS.Analytical chemistry · 2025Article
- Deciphering Colorectal Cancer-Hepatocyte Interactions: A Multiomics Platform for Interrogation of Metabolic Crosstalk in the Liver-Tumor Microenvironment.International journal of molecular sciences · 2025Article
- Distinguishing Artifactual Fatty Acid Dimers from Fatty Acid Esters of Hydroxy Fatty Acids in Untargeted LC-MS Pipelines.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Distinguishing Artifactual Fatty Acid Dimers from Fatty Acid Esters of Hydroxy Fatty Acids in Untargeted LC-MS Pipelines.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Exercise improves systemic metabolism in a monocrotaline model of pulmonary hypertension.Sports medicine and health science · 2025Article
- Advancements in Mass Spectrometry-Based Targeted Metabolomics and Lipidomics: Implications for Clinical Research.Molecules (Basel, Switzerland) · 2024Review
- Deciphering Colorectal Cancer-Hepatocyte Interactions: A Multiomic Platform for Interrogation of Metabolic Crosstalk in the Liver-Tumor Microenvironment.bioRxiv : the preprint server for biology · 2024Article
- A diverse proteome is present and enzymatically active in metabolite extracts.Nature communications · 2024Article
- Ferrous ascorbate as a potential biomarker for diabetic retinopathy: a vitreous humour metabolomics study.BMC ophthalmology · 2024Article
- Improvement and prediction of the extraction parameters of lupeol and stigmasterol metabolites of Melia azedarach with response surface methodology.BMC biotechnology · 2024Article
- Preparation of high-efficiency HILIC capillary columns utilizing slurry packing at 2100 bar.Journal of chromatography. A · 2024Article
- Integrative proteome and metabolome analyses reveal molecular basis of the tail resorption during the metamorphic climax ofFrontiers in cell and developmental biology · 2024Article
77 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Although the objective of any 'omic science is broad measurement of its constituents, such coverage has been challenging in metabolomics because the metabolome is comprised of a chemically diverse set of small molecules with variable physical properties. While extensive studies have been performed to identify metabolite isolation and separation methods, these strategies introduce bias toward lipophilic or water-soluble metabolites depending on whether reversed-phase (RP) or hydrophilic interaction liquid chromatography (HILIC) is used, respectively. Here we extend our consideration of metabolome isolation and separation procedures to integrate RPLC/MS and HILIC/MS profiling. An aminopropyl-based HILIC/MS method was optimized on the basis of mobile-phase additives and pH, followed by evaluation of reproducibility. When applied to the untargeted study of perturbed bacterial metabolomes, the HILIC method enabled the accurate assessment of key, dysregulated metabolites in central carbon pathways (e.g., amino acids, organic acids, phosphorylated sugars, energy currency metabolites), which could not be retained by RPLC. To demonstrate the value of the integrative approach, bacterial cells, human plasma, and cancer cells were analyzed by combined RPLC/HILIC separation coupled to ESI positive/negative MS detection. The combined approach resulted in the observation of metabolites associated with lipid and central carbon metabolism from a single biological extract, using 80% organic solvent (ACN:MeOH:H2O 2:2:1). It enabled the detection of more than 30,000 features from each sample type, with the highest number of uniquely detected features by RPLC in ESI positive mode and by HILIC in ESI negative mode. Therefore, we conclude that when time and sample are limited, the maximum amount of biological information related to lipid and central carbon metabolism can be acquired by combining RPLC ESI positive and HILIC ESI negative mode analysis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.