ArticleEvidence-based complementary and alternative medicine : eCAM2013
Inhibition of Inflammatory Response by Artepillin C in Activated RAW264.7 Macrophages.
Article in Evidence-based complementary and alternative medicine : eCAM, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 54 citations in OpenAlex.
- Propolis Stands out as a Multifaceted Natural Product: Meta-Analysis on Its Sources, Bioactivities, Applications, and Future Perspectives.Life (Basel, Switzerland) · 2025Review
- Artepillin C Time-Dependently Alleviates Metabolic Syndrome in Obese Mice by Regulating CREB/CRTC2-BMAL1 Signaling.Nutrients · 2023Article
- Propolis polyphenols: A review on the composition and anti-obesity mechanism of different types of propolis polyphenols.Frontiers in nutrition · 2023Review
- Investigating the cellular antioxidant and anti-inflammatory effects of the novel peptides in lingzhi mushrooms.Heliyon · 2022Article
- Propolis: Its Role and Efficacy in Human Health and Diseases.Molecules (Basel, Switzerland) · 2022Review
- Propolis: An update on its chemistry and pharmacological applications.Chinese medicine · 2022Review
- Molecular Insights into the Antistress Potentials of Brazilian Green Propolis Extract and Its Constituent Artepillin C.Molecules (Basel, Switzerland) · 2021Article
- Artepillin C Reduces Allergic Airway Inflammation by Induction of Monocytic Myeloid-Derived Suppressor Cells.Pharmaceutics · 2021Article
- Immunomodulatory Effect of Propolis on Foxp3 Gene Expression in Human Peripheral Blood Mononuclear Cells StimulatedArchives of Razi Institute · 2021Article
- Brazilian green propolis promotes TNFR2 expression on regulatory T cells.Food science & nutrition · 2021Article
- The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury.Molecules (Basel, Switzerland) · 2020Article
- Antioxidant and anti-inflammatory peptide fraction from oyster soft tissue by enzymatic hydrolysis.Food science & nutrition · 2020Article
- Effects of Propolis and Phenolic Acids on Triple-Negative Breast Cancer Cell Lines: Potential Involvement of Epigenetic Mechanisms.Molecules (Basel, Switzerland) · 2020Article
- Direct Biomarkers of Microbial Translocation Correlate with Immune Activation in Adult Zambians with Environmental Enteropathy and Hepatosplenic Schistosomiasis.The American journal of tropical medicine and hygiene · 2017Article
- Antioxidant Activity of SOD and Catalase Conjugated with Nanocrystalline Ceria.Bioengineering (Basel, Switzerland) · 2017Article
- Parasite Killing ofEvidence-based complementary and alternative medicine : eCAM · 2017Article
- A herbal formula comprising Rosae Multiflorae Fructus and Lonicerae Japonicae Flos, attenuates collagen-induced arthritis and inhibits TLR4 signalling in rats.Scientific reports · 2016Article
- Chemical Characterization and Antioxidant, Antimicrobial, and Anti-Inflammatory Activities of South Brazilian Organic Propolis.PloS one · 2016Article
- The assessment of inflammatory activity and toxicity of treated sewage using RAW264.7 cells.Water and environment journal : the journal · 2015Article
- Biological activities and cytotoxicity of diterpenes from Copaifera spp. Oleoresins.Molecules (Basel, Switzerland) · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) is the main bioactive component of Brazilian green propolis. The aim of this study was to investigate the anti-inflammatory effect of artepillin C on LPS + IFN- γ - or PMA-stimulated RAW264.7 macrophages. The cell viability was evaluated by MTT and LDH assays. The radical scavenging ability was determined using DPPH(•) and ABTS(•+). ROS and RNS generation was analyzed by chemiluminescence. NO concentration was detected by the Griess reaction. The release of various cytokines by activated RAW264.7 cells was measured in the culture supernatants using a multiplex bead array system based on xMAP technology. NF- κ B activity was confirmed by the ELISA-based TransAM NF- κ B kit. At the tested concentrations, the compound did not decrease the cell viability and did not cause the cytotoxicity. Artepillin C exerted strong antioxidant activity, significantly inhibited the production of ROS, RNS, NO, and cytokine IL-1 β , IL-3, IL-4, IL-5, IL-9, IL-12p40, IL-13, IL-17, TNF- α , G-CSF, GM-CSF, MCP-1, MIP-1 α , MIP-1 β , RANTES, and KC, and markedly blocked NF- κ B expression in stimulated RAW264.7 macrophages. Our findings provide new insights for understanding the mechanism involved in the anti-inflammatory effect of artepillin C and support the application of Brazilian green propolis in complementary and alternative medicine.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.