ArticleFrontiers in genetics2013
Green fluorescent protein-based monitoring of endoplasmic reticulum redox poise.
Article in Frontiers in genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- Endoplasmic Reticulum Redoxome: Protein Folding and Beyond.Biochemistry · 2026Review
- Endoplasmic reticulum oxidoreductin provides resilience against reductive stress and hypoxic conditions by mediating luminal redox dynamics.The Plant cell · 2022Article
- Endoplasmic Reticulum Stress and Reactive Oxygen Species in Plants.Antioxidants (Basel, Switzerland) · 2022Review
- CaBMC biology · 2020Article
- Variety is the spice of life: how to explore a redox-dependent heterogeneity in genomically identical cellular populations.Current genetics · 2019Article
- Stable Integration and Comparison of hGrx1-roGFP2 and sfroGFP2 Redox Probes in the Malaria Parasite Plasmodium falciparum.ACS infectious diseases · 2018Article
- Hexose-6-phosphate dehydrogenase controls cancer cell proliferation and migration through pleiotropic effects on the unfolded-protein response, calcium homeostasis, and redox balance.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- ATP sensing in living plant cells reveals tissue gradients and stress dynamics of energy physiology.eLife · 2017Article
- TriPer, an optical probe tuned to the endoplasmic reticulum tracks changes in luminal HBMC biology · 2017Article
- Redox- and Reactive Oxygen Species-Dependent Signaling into and out of the Photosynthesizing Chloroplast.Plant physiology · 2016Review
- Measuring Redox Status of Melanoma Cells.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Cysteines 208 and 241 in Ero1α are required for maximal catalytic turnover.Redox biology · 2016Article
- Oxidative Stress during HIV Infection: Mechanisms and Consequences.Oxidative medicine and cellular longevity · 2016Review
- Retarded PDI diffusion and a reductive shift in poise of the calcium depleted endoplasmic reticulum.BMC biology · 2015Article
- Endoplasmic reticulum stress response and transcriptional reprogramming.Frontiers in genetics · 2014Article
- Endoplasmic reticulum oxidoreductin-1α (Ero1α) improves folding and secretion of mutant proinsulin and limits mutant proinsulin-induced endoplasmic reticulum stress.The Journal of biological chemistry · 2013Article
- Destroy and exploit: catalyzed removal of hydroperoxides from the endoplasmic reticulum.International journal of cell biology · 2013Review
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pathological endoplasmic reticulum (ER) stress is tightly linked to the accumulation of reactive oxidants, which can be both upstream and downstream of ER stress. Accordingly, detrimental intracellular stress signals are amplified through establishment of a vicious cycle. An increasing number of human diseases are characterized by tissue atrophy in response to ER stress and oxidative injury. Experimental monitoring of stress-induced, time-resolved changes in ER reduction-oxidation (redox) states is therefore important. Organelle-specific examination of redox changes has been facilitated by the advent of genetically encoded, fluorescent probes, which can be targeted to different subcellular locations by means of specific amino acid extensions. These probes include redox-sensitive green fluorescent proteins (roGFPs) and the yellow fluorescent protein-based redox biosensor HyPer. In the case of roGFPs, variants with known specificity toward defined redox couples are now available. Here, we review the experimental framework to measure ER redox changes using ER-targeted fluorescent biosensors. Advantages and drawbacks of plate-reader and microscopy-based measurements are discussed, and the power of these techniques demonstrated in the context of selected cell culture models for ER stress.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.