ArticleJournal of gastroenterology2014
The possible roles of hyperpolarization-activated cyclic nucleotide channels in regulating pacemaker activity in colonic interstitial cells of Cajal.
Article in Journal of gastroenterology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- Changrun Formula Relieves Functional Constipation by Improving Intestinal Motility in Rats.Gastroenterology research and practice · 2025Article
- Hyperpolarization-activated cyclic nucleotide-gated channels working as pacemaker channels in colonic interstitial cells of Cajal.Journal of cellular and molecular medicine · 2022Article
- Moxibustion Regulates Gastrointestinal Motility via HCN1 in Functional Dyspepsia Rats.Medical science monitor : international medical journal of experimental and clinical research · 2021Article
- The effect of Xenin25 on spontaneous circular muscle contractions of rat distal colon in vitro.Physiological reports · 2021Article
- Na-K-2Cl Cotransporter and Store-Operated CaBiophysical journal · 2019Article
- HCN as a Mediator of Urinary Homeostasis: Age-Associated Changes in Expression and Function in Adrenergic Detrusor Relaxation.The journals of gerontology. Series A, Biological sciences and medical sciences · 2019Article
- Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels: An Emerging Role in Neurodegenerative Diseases.Frontiers in molecular neuroscience · 2019Review
- Consuming Genistein Improves Survival Rates in the Absence of Laxative in ΔF508-CF Female Mice.Nutrients · 2018Article
- Decreased hyperpolarization-activated cyclic nucleotide-gated channels are involved in bladder dysfunction associated with spinal cord injury.International journal of molecular medicine · 2018Article
- Hyperpolarization-Activated Cyclic Nucleotide-Gated Non-selective (HCN) Ion Channels Regulate Human and Murine Urinary Bladder Contractility.Frontiers in physiology · 2018Article
- Loss of HCN2 leads to delayed gastrointestinal motility and reduced energy intake in mice.PloS one · 2018Article
- Prostanoid EP3 receptor agonist sulprostone enhances pacemaker activity of colonic interstitial cells of Cajal.Naunyn-Schmiedeberg's archives of pharmacology · 2017Article
- Cyclophosphamide-induced HCN1 channel upregulation in interstitial Cajal-like cells leads to bladder hyperactivity in mice.Experimental & molecular medicine · 2017Article
- Association between hyperpolarization-activated channel in interstitial cells of Cajal and gastrointestinal dysmotility induced by malignant ascites.Oncology letters · 2017Article
- Transcriptome of interstitial cells of Cajal reveals unique and selective gene signatures.PloS one · 2017Article
- NPs/NPRs Signaling Pathways May Be Involved in Depression-Induced Loss of Gastric ICC by Decreasing the Production of mSCF.PloS one · 2016Article
- Decreased expression of hyperpolarisation-activated cyclic nucleotide-gated channel 3 in Hirschsprung's disease.World journal of gastroenterology · 2015Article
- Basal cGMP regulates the resting pacemaker potential frequency of cultured mouse colonic interstitial cells of Cajal.Naunyn-Schmiedeberg's archives of pharmacology · 2014Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHyperpolarization-activated cyclic nucleotide (HCN) channels are pacemaker channels that regulate heart rate and neuronal rhythm in spontaneously active cardiac and neuronal cells. Interstitial cells of Cajal (ICCs) are also spontaneously active pacemaker cells in the gastrointestinal tract. Here, we investigated the existence of HCN channel and its role on pacemaker activity in colonic ICCs.
methodsWe performed whole-cell patch clamp, RT-PCR, and Ca(2+)-imaging in cultured ICCs from mouse mid colon.
resultsSQ-22536 and dideoxyadenosine (adenylate cyclase inhibitors) decreased the frequency of pacemaker potentials, whereas both rolipram (cAMP-specific phosphodiesterase inhibitor) and cell-permeable 8-bromo-cAMP increased the frequency of pacemaker potentials. CsCl, ZD7288, zatebradine, clonidine (HCN channel blockers), and genistein (a tyrosine kinase inhibitor) suppressed the pacemaker activity. RT-PCR revealed expression of HCN1 and HCN3 channels in c-kit and Ano1 positive colonic ICCs. In recordings of spontaneous intracellular Ca(2+) [Ca(2+)]i oscillations, rolipram and 8-bromo-cAMP increased [Ca(2+)]i oscillations, whereas SQ-22536, CsCl, ZD7288, and genistein decreased [Ca(2+)]i oscillations.
conclusionsHCN channels in colonic ICCs are tonically activated by basal cAMP production and participate in regulation of pacemaking activity.
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