ArticleMemorias do Instituto Oswaldo Cruz2013
Multiplex polymerase chain reaction to identify and determine the toxigenicity of Corynebacterium spp with zoonotic potential and an overview of human and animal infections.
Article in Memorias do Instituto Oswaldo Cruz, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 48 citations in OpenAlex.
- Genomic regulation of the diphtheria toxin gene and Its implications for molecular diagnostics and surveillance in low-resource settings.Molecular biology reports · 2026Review
- Caseous Lymphadenitis Outbreak in Dairy Cattle: Clinical Findings, Management, and Autogenous Vaccine Development.Veterinary sciences · 2025Article
- Challenges of Diphtheria Toxin Detection.Toxins · 2024Review
- Rising prevalence and drug resistance ofFrontiers in cellular and infection microbiology · 2024Review
- Update on Novel Taxa and Revised Taxonomic Status of Bacteria Isolated from Nondomestic Animals Described in 2018 to 2021.Journal of clinical microbiology · 2023Review
- Epidemiological Investigation of Hospital Transmission of Corynebacterium striatum Infection by Core Genome Multilocus Sequence Typing Approach.Microbiology spectrum · 2023Article
- Using the PCR and Blood Agar in Diagnosis of Semen Bacterial Contamination of Fertile and Infertile Men.Reports of biochemistry & molecular biology · 2021Article
- Molecular Characterization ofVeterinary sciences · 2021Article
- Worldwide survey of Corynebacterium striatum increasingly associated with human invasive infections, nosocomial outbreak, and antimicrobial multidrug-resistance, 1976-2020.Archives of microbiology · 2021Review
- Bacteriological, cytological, and molecular investigation of Corynebacterium pseudotuberculosis, mycobacteria, and other bacteria in caseous lymphadenitis and healthy lymph nodes of slaughtered sheep.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Article
- Cutaneous infection by non-diphtheria-toxin producing and penicillin-resistantAccess microbiology · 2021Article
- Utility assessment of an Enzyme-linked immunosorbent assay for detection of subclinical cases of caseous lymphadenitis in small ruminant flocks.Veterinary medicine and science · 2020Article
- Phylogenomic characterisation of a novel corynebacterial species pathogenic to animals.Antonie van Leeuwenhoek · 2020Article
- Corynebacterium tuberculostearicum, a human skin colonizer, induces the canonical nuclear factor-κB inflammatory signaling pathway in human skin cells.Immunity, inflammation and disease · 2020Article
- Searching whole genome sequences for biochemical identification features of emerging and reemerging pathogenic Corynebacterium species.Functional & integrative genomics · 2018Article
- Comparative genomic analysis between Corynebacterium pseudotuberculosis strains isolated from buffalo.PloS one · 2017Article
Corrections and comments
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Authors and funding
13 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Corynebacterium diphtheriae, Corynebacterium ulcerans and Corynebacterium pseudotuberculosis constitute a group of potentially toxigenic microorganisms that are related to different infectious processes in animal and human hosts. Currently, there is a lack of information on the prevalence of disease caused by these pathogens, which is partially due to a reduction in the frequency of routine laboratory testing. In this study, a multiplex polymerase chain reaction (mPCR) assay that can simultaneously identify and determine the toxigenicity of these corynebacterial species with zoonotic potential was developed. This assay uses five primer pairs targeting the following genes: rpoB (Corynebacterium spp), 16S rRNA (C. ulcerans and C. pseudotuberculosis), pld (C. pseudotuberculosis), dtxR (C. diphtheriae) and tox [diphtheria toxin (DT) ]. In addition to describing this assay, we review the literature regarding the diseases caused by these pathogens. Of the 213 coryneform strains tested, the mPCR results for all toxigenic and non-toxigenic strains of C . diphtheriae, C. ulcerans and C. pseudotuberculosis were in 100% agreement with the results of standard biochemical tests and PCR-DT. As an alternative to conventional methods, due to its advantages of specificity and speed, the mPCR assay used in this study may successfully be applied for the diagnosis of human and/or animal diseases caused by potentially toxigenic corynebacterial species.
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Registered trials
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