ArticleMolecular pharmacology2013
Segregation of family A G protein-coupled receptor protomers in the plasma membrane.
Article in Molecular pharmacology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Interclass GPCR heteromerization affects localization and trafficking.Science signaling · 2020Article
- Article
- Serotonin and Glutamate Interactions in Preclinical Schizophrenia Models.ACS chemical neuroscience · 2019Review
- Regulation of G protein-coupled receptor signaling by plasma membrane organization and endocytosis.Traffic (Copenhagen, Denmark) · 2019Review
- Conformational biosensors reveal allosteric interactions between heterodimeric AT1 angiotensin and prostaglandin F2α receptors.The Journal of biological chemistry · 2017Article
- Spatial intensity distribution analysis quantifies the extent and regulation of homodimerization of the secretin receptor.The Biochemical journal · 2017Article
- Organization and functions of mGlu and GABANature · 2016Review
- Referenced Single-Molecule Measurements Differentiate between GPCR Oligomerization States.Biophysical journal · 2015Article
- Distinct Agonist Regulation of Muscarinic Acetylcholine M2-M3 Heteromers and Their Corresponding Homomers.The Journal of biological chemistry · 2015Article
- Regulation of oligomeric organization of the serotonin 5-hydroxytryptamine 2C (5-HT2C) receptor observed by spatial intensity distribution analysis.The Journal of biological chemistry · 2015Article
- BRET evidence that β2 adrenergic receptors do not oligomerize in cells.Scientific reports · 2015Article
- CrossTalk opposing view: Weighing the evidence for class A GPCR dimers, the jury is still out.The Journal of physiology · 2014Article
- Family a GPCR heteromers in animal models.Frontiers in pharmacology · 2014Article
- G protein-coupled receptor oligomerization revisited: functional and pharmacological perspectives.Pharmacological reviews · 2014Review
- Taking two to tango: a role for ghrelin receptor heterodimerization in stress and reward.Frontiers in neuroscience · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
G protein-coupled receptors (GPCRs) transduce many important physiological signals and are targets for a large fraction of therapeutic drugs. Members of the largest family of GPCRs (family A) are thought to self-associate as dimers and higher-order oligomers, although the significance of such quaternary structures for signaling or receptor trafficking is known for only a few examples. One outstanding question is the physical stability of family A oligomers in cell membranes. Stable oligomers would be expected to move through cellular compartments and membrane domains as intact groups of protomers. Here, we test this prediction by recruiting subsets of affinity-tagged family A protomers into artificial microdomains on the surface of living cells and asking if untagged protomers move into these domains (are corecruited) at the same time. We find that tagged β₂ adrenergic and μ-opioid protomers are unable to corecruit untagged protomers into microdomains. In contrast, tagged metabotropic glutamate receptor protomers do corecruit untagged protomers into such microdomains, which is consistent with the known covalent mechanism whereby these family C receptors dimerize. These observations suggest that interactions between these family A protomers are too weak to directly influence subcellular location, and that mechanisms that move these receptors between subcellular compartments and domains must operate on individual protomers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.