ArticleInternational journal of applied & basic medical research2013
An increase of granulosa cell apoptosis mediates aqueous neem (Azadirachta indica) leaf extract-induced oocyte apoptosis in rat.
Article in International journal of applied & basic medical research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 66 citations in OpenAlex.
- Ovarian toxic effects of micro- and nanoplastics (Review).Experimental and therapeutic medicine · 2026Review
- Research Progress on Mammalian Oocyte Vitrification: From Damage Mechanisms to Optimization Strategies.Animals : an open access journal from MDPI · 2026Review
- Polystyrene microplastics and di-2-ethylhexyl phthalate co-exposure: Implications for female reproductive health.Environmental science and ecotechnology · 2024Article
- Single-cell RNA-seq reveals the effects of the FecB mutation on the transcriptome profile in ovine cumulus cells.Scientific reports · 2024Article
- Honghua Xiaoyao tablet combined with estradiol improves ovarian function in D-galactose-induced aging mice by reducing apoptosis and affecting the release of reproductive hormones: anFrontiers in pharmacology · 2024Article
- The Dose-Related Efficacy of Human Placenta-Derived Mesenchymal Stem Cell Transplantation on Antioxidant Effects in a Rat Model with Ovariectomy.Antioxidants (Basel, Switzerland) · 2023Article
- EIF4A3-Induced Exosomal circLRRC8A Alleviates Granulosa Cells Senescence Via the miR-125a-3p/NFE2L1 axis.Stem cell reviews and reports · 2023Article
- A novel model based on necroptosis to assess progression for polycystic ovary syndrome and identification of potential therapeutic drugs.Frontiers in endocrinology · 2023Article
- hsa-miR-320a-3p and hsa-miR-483-5p levels in human granulosa cells: promising bio-markers of live birth after IVF/ICSI.Reproductive biology and endocrinology : RB&E · 2022Article
- Plant-Derived Natural Compounds for Tick Pest Control in Livestock and Wildlife: Pragmatism or Utopia?Insects · 2020Review
- The Role of Oxidative Stress and Natural Antioxidants in Ovarian Aging.Frontiers in pharmacology · 2020Review
- Autophagy in hypoxic ovary.Cellular and molecular life sciences : CMLS · 2019Review
- Necroptosis in stressed ovary.Journal of biomedical science · 2019Review
- An Overview on the Anticancer Activity ofInternational journal of molecular sciences · 2018Review
- Melatonin Promotes Ubiquitination of Phosphorylated Pro-Apoptotic Protein Bcl-2-Interacting Mediator of Cell Death-Extra Long (BimInternational journal of molecular sciences · 2018Article
- Germ cell depletion from mammalian ovary: possible involvement of apoptosis and autophagy.Journal of biomedical science · 2018Review
- A Biochemical Approach to Detect Oxidative Stress in Infertile Women Undergoing Assisted Reproductive Technology Procedures.International journal of molecular sciences · 2018Article
- Knockdown of XBP1 by RNAi in Mouse Granulosa Cells Promotes Apoptosis, Inhibits Cell Cycle, and Decreases Estradiol Synthesis.International journal of molecular sciences · 2017Article
- Article
- The Role of Antioxidant Enzymes in the Ovaries.Oxidative medicine and cellular longevity · 2017Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveNeem plant (Azadirachta indica) has been extensively used in Ayurvedic system of medicine for female fertility regulation for a long time, but its mechanism of action remains poorly understood. Hence, the present study was aimed to determine whether an increase of granulosa cell apoptosis is associated with aqueous neem leaf extract (NLE)-induced oocyte apoptosis. MATERIALS AND
methodsSexually immature female rats of 20 days old were fed NLE (50 mg/day) for 10 days and then subjected to superovulation induction protocol. The morphological changes in cumulus oocyte complexes (COCs), rate of oocyte apoptosis, hydrogen peroxide (H2O2), total nitrite, and cytochrome c concentrations, inducible nitric oxide synthase (iNOS), cytochrome c, p53, Bcl2 and Bax expressions, deoxyribonucleic acid (DNA) fragmentation, and estradiol 17β level in granulosa cells collected from preovulatory COCs were analyzed.
resultsAqueous NLE increased H2O2 concentration and decreased catalase activity, increased iNOS expression and total nitrite concentration, increased p53, Bax, and p53 expressions but decreased Bcl2 expression, increased cytochrome c concentration and induced DNA fragmentation in granulosa cells. An increased granulosa cell apoptosis resulted in reduced estradiol 17β concentration and induced apoptosis in ovulated oocytes.
conclusionWe conclude that aqueous NLE-induced granulosa cell apoptosis through the mitochondria-mediated pathway, reduced estradiol 17β concentration and induced apoptosis in ovulated oocytes. Thus, granulosa cell apoptosis mediates NLE-induced oocyte apoptosis during female fertility regulation in rat.
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